Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Mops (sodium salt) | 71119-22-7 | 99.83% | 10 G
The product is a buffering agent utilized in biology. It serves to maintain the pH of mammalian cell culture media and notably enhances the thermal stability of BSA.
- Functions as a buffering agent in biological applications.
- Maintains the pH level of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Can be used in the preparation of keratinocyte and microbial culture medium.
- Relatively safe for short-term experiments, though prolonged use may reduce cell viability.
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 6X500G
MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 6X500G
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-25.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 8.0) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Medchemexpress LLC Bicine | 150-25-4 | 98.7% | 25 G
Bicine is a buffer utilized in physiological research. It is a derivative of the amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection.
- Buffer utilized in physiological research
- Derivative of the amino acid glycine
- Enhances the sensitivity of guanosine enzyme histochemical detection
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | C29H33N3O9S2 | 10 MG
AR-13324 analog mesylate is a research-grade mesylate salt analog of AR-13324, a Rho-associated kinase (ROCK) and norepinephrine transporter (NET) inhibitor. It is supplied as a light brown to brown liquid intended for laboratory research and assay development.
- Research-use compound related to ROCK and NET inhibition.
- High purity (98.0%) suitable for analytical and biological assays.
- Molecular weight 631.72 g/mol for formulation calculations.
- Supplied as a mesylate salt in a liquid form for easy handling.
- Store sealed, away from moisture; in solvent: -80°C for 6 months, -20°C for 1 month.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 2.5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659991.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 2.5 Kg
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a highly effective biochemical reagent used for pH buffering in a variety of biological, biochemical, and chemical processes. This 2.5 kg container is ideal for high-throughput laboratories that require a stable, consistent pH environment. MOPS is commonly used in experiments related to protein and nucleic acid analysis, enzyme activity assays, and electrophoresis. Its ability to maintain a steady pH of 7.0–7.5 makes it indispensable in cell culture and biological research where pH fluctuations could interfere with sensitive processes. It is also a standard reagent for maintaining pH in buffers for molecular biology applications.
Gold Biotechnology Inc MOPS, Sodium Salt 1 kg
MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
Research Products International Corp PIPES DISODIUM SALT, 100G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 50 MG
AR-13324 analog mesylate is an analog of AR-13324. AR-13324 is an ROCK and norepinephrine transporter (NET) inhibitor.
- Analog of AR-13324.
- Ar-13324 is a rock and norepinephrine transporter (net) inhibitor.
- Available in various sizes, including 50 mg.
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659862.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is a biochemical reagent widely used in laboratory buffer solutions. This 100g formulation is perfect for maintaining a stable pH in various biological and biochemical assays. The disodium salt form provides a slightly stronger buffering capacity compared to its other forms, making it ideal for sensitive experiments such as protein electrophoresis and enzyme activity assays. It helps maintain a consistent pH of approximately 7.0, which is essential for stabilizing biological molecules during lab procedures. PIPES disodium salt is a reliable buffer in cell culture, microbiology, and biochemical studies.
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 250G
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 250G
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 1KG
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 1KG
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-50.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.