Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPSO >=99% (titration) | 68399-77-9 | MFCD00009629 | 100G
MOPSO >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 225.26 | 68399-77-9 | MFCD00009629 | 100G
Biotang Inc Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Research Products International Corp ACES, 5 KG
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
Biotang Inc Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
Biotang Inc TRIS-Buffered Saline (10X, Low Salt), 1000 ml
TRIS-Buffered Saline (10X, Low Salt), 1000 ml.
Biotang Inc Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml
Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml.
Medchemexpress LLC HEPES (sodium) | 75277-39-3 | MFCD00036463 | 99.9% | 260.29 | 250 MG
HEPES sodium is a nonvolatile zwitterionic chemical buffering agent broadly applied in cell culture. It is effective at pH 6.8 to 8.2 and is also a potent inducer of lysosome biogenesis.
- Nonvolatile zwitterionic chemical buffering agent
- Broadly applied in cell culture
- Effective at pH 6.8 to 8.2
- Potent inducer of lysosome biogenesis
- Maintains superhydrophilicity of titanium for at least 3 months, resulting in continuous retention of bioactivity and osteoconductivity
- Drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, and disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells