Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 1KG
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-500.04.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-500.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 1KG
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 1KG
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-500.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 500 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Research Products International Corp PIPES FREE ACID ULTRAPURE, 10GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp TE Buffer, 10X Powder, 158.35 Grams of Powder, Makes 10 Liters of Solution
Dry homogeneous blend of molecular biology grade Tris-base and EDTA, disodium salt. For use in gel electrophoresis.
Supplied as 158.35 grams of powder which is used to produce a 10X concentrate.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Teknova 1X PBS with 0.05% Sodium Azide. 2L, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ MOPS Buffer 10X, Amber Bottle, 1L
Alkali Scientific™ CellPro™ MOPS Buffer 10X is a versatile reagent commonly used in molecular biology and biochemistry applications. With its optimized formulation, this buffer provides stable pH conditions, crucial for nucleic acid electrophoresis, RNA isolation, and protein gel electrophoresis. Each batch undergoes stringent sterile filtration to ensure purity and consistency, making CellPro™ MOPS Buffer 10X a trusted choice for researchers seeking reliable results in their experiments.
Alkali Scientific CellPro™ Sterile Saline, 0.9%, 1L, 10/cs
Introducing CellPro™ Sterile Saline, meticulously crafted by Alkali Scientific™ to meet the demanding standards of molecular biology applications. This Molecular Biology Grade saline solution ensures exceptional purity and sterility, making it ideal for various laboratory procedures, including cell culture, molecular assays, and biochemical experiments. With its precisely balanced formulation, CellPro™ Sterile Saline provides the essential ions and osmotic balance required for cell viability and physiological studies. Each batch undergoes rigorous quality control to guarantee consistency and reliability, empowering researchers with confidence in their results. Trust CellPro™ Sterile Saline to support your molecular biology research with unparalleled quality and performance.
Alkali Scientific CellPro™ Tris HCl, 1M, pH 8.0, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Research Products International Corp Blot Stripping Buffer 4X Solution, 250 ML
Product Formulation: TRIS-HCl: 250 mM; SDS: 8%; pH: 6.8. Add mercaptoethanol according to protocol.
Instructions :
1. Dilute 4X Stripping Bufffer ( 1part Stripping Buffer: 3 parts distilled water) and add beta-mercaptoethanol (0.6%).
2. Wash the wet membrane in the stripping buffer for 5 minutes.
3. Incubate the membrane in stripping buffer for 30 minutes at 65 degrees Celius while agitating.