Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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3,421 – 3,435 of 3,489 results

3,421

Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.0) - 500ml

The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.

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3,422

Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 4L

Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications. This product functions as wash buffer in Western blotting and immunoassays, and is formulated in a 10X concentration. Dilute to desired concentration.

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3,423

Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK

Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK

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3,424

FUJIFILM Biosciences PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid

PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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3,425

Bioworld MES Buffer 1M, pH 6.1n 4432-31-9, 250ml

Also known as [2-(N-Morpholino) ethanesulfonic Acid]. MES-Buffer has a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. A biological buffer mainly used in plant cell cultures. Technical Information: Used for culture media. Ultra-Pure Grade, pKa (at 25°C): 6.10+/-0.05, pH 6.1, Molecular Weight : 195.24, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : >300 °C(lit.) 250ml

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3,426

Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 4L

Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics physiological osmolarity and ion concentrations, making it ideal for applications including protein purification, Western blotting and immunoassays, sample dilution, and as a transport medium. Packaged as a 10X concentration, this solution can be diluted to support its application.

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3,427

Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set Crystals, 250 g

Trizma Pre-set Crystals, 250 g.

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3,428

BIO TECHNE SALES CORPORATION TURBO CE-SDS RUNNING BUFFER

NC2144853 TURBO CE-SDS RUNNING BUFFER

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3,429

PIPES, Sesquisodium Sodium Salt, Fisher BioReagents

CAS: 100037-69-2 Molecular Formula: C16H33N4Na3O12S4 Molecular Weight (g/mol): 670.665 InChI Key: FPDACHMSOMILQI-UHFFFAOYSA-K Synonym: 1, 4-Piperazinediethanesulfonic acid sesquisodium salt,Piperazine-1, 4-bis(2-ethanesulfonic acid) sesquisodium salt PubChem CID: 3086462 IUPAC Name: trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+]

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Specifications

PubChem CID	3086462
CAS	100037-69-2
Molecular Weight (g/mol)	670.665
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+]
Synonym	1, 4-Piperazinediethanesulfonic acid sesquisodium salt,Piperazine-1, 4-bis(2-ethanesulfonic acid) sesquisodium salt
IUPAC Name	trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate
InChI Key	FPDACHMSOMILQI-UHFFFAOYSA-K
Molecular Formula	C16H33N4Na3O12S4

3,430

TE Buffer, Tris-EDTA, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA (TE), 10X Solution
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter and autoclaved.
ChemAlert Storage Symbol	Gray
Name Note	pH 7.4
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

3,431

TAE Buffer, Tris-Acetate-EDTA, 25X Powder, Electrophoresis, Fisher BioReagents

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3,432

Fisher Scientific TES (White Crystals), Fisher BioReagents

CAS: 7365-44-8 Molecular Formula: C6H15NO6S Molecular Weight (g/mol): 229.247 InChI Key: JOCBASBOOFNAJA-UHFFFAOYSA-N Synonym: N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid PubChem CID: 81831 ChEBI: CHEBI:44356 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NC(CO)(CO)CO

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Specifications

PubChem CID	81831
CAS	7365-44-8
Molecular Weight (g/mol)	229.247
ChEBI	CHEBI:44356
SMILES	C(CS(=O)(=O)O)NC(CO)(CO)CO
Synonym	N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid
InChI Key	JOCBASBOOFNAJA-UHFFFAOYSA-N
Molecular Formula	C6H15NO6S

3,433

Tris(hydroxymethyl)aminomethane (Crystalline/Laboratory), Fisher Chemical™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: THAM,2-Amino-2-(hydroxymethyl)-1, 3-Propanediol Trimethylol Aminomethane,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	THAM,2-Amino-2-(hydroxymethyl)-1, 3-Propanediol Trimethylol Aminomethane,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

3,434

TE Buffer, Tris-EDTA, 10X Solution, pH 7.6, Molecular Biology, Fisher BioReagents™

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA (TE), 10X Solution
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter and autoclaved.
ChemAlert Storage Symbol	Gray
Name Note	pH 7.6
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

3,435

Fisher Scientific MOPS 10X Solution, Fisher BioReagents™

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-n-morpholinopropanesulfonic acid,4-morpholinepropane sulfonic acid,3-morpholinopropanesulfonic acid,3-morpholinopropane-1-sulfonic acid,morpholinopropane sulfonic acid,unii-273bp63nv3,3-n-morpholino propanesulfonic acid,morpholinopropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid,4-morpholinepropanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 4-(3-sulfonatopropyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	3-n-morpholinopropanesulfonic acid,4-morpholinepropane sulfonic acid,3-morpholinopropanesulfonic acid,3-morpholinopropane-1-sulfonic acid,morpholinopropane sulfonic acid,unii-273bp63nv3,3-n-morpholino propanesulfonic acid,morpholinopropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid,4-morpholinepropanesulfonic acid
IUPAC Name	4-(3-sulfonatopropyl)morpholin-4-ium
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

Life Science Buffers

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Fisher Scientific TES (White Crystals), Fisher BioReagents GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Fisher Scientific TES (White Crystals), Fisher BioReagents