Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
STEMCELL Technologies D-PBS (Without Ca++ and Mg++), Size: 500 mL
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 100mL
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Rockland Immunochemicals 10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2) - MB-075-1000
Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.
Supply Solutions Trizma Pre-set crystals, 250g
BioPerformance Certified, pH 7.0, average Mw 154.8, Synonym: Tris crystals
ProteinSimple Maurice CE-SDS Running Buffer - Bottom
Running buffer for CE-SDS application. 15 mL. For use with CE-SDS cartridge (PS-MC02-S) on Maurice (090-000) and Maurice S. (090-001) systems only.
STEMCELL Technologies RoboSep™ Buffer, Size: 250 mL
RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Teknova 20X PBS with 1% Tween-20, pH 7.3 (1000mL, Sterile)
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Polysciences, Inc. 1M Glycine-HCl Buffer, pH 2.7±0.1, 10X Concentrate 500 ML
This item has a minimum qty of 2 per supplier requirements.
1M GLYCINE-HCL BUFFER 500 ML
Supply Solutions Red Blood Cell Lysing Buffer Hybri-Max™, 100mL
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 10/pk
Phosphate buffered saline | MFCD00131855 | 10/pk
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.