Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Lonza Walkersville Tris Buffer 50 mM
Tris buffer can be used in place of water as the sample diluent for highly acidic or basic samples (for endotoxin testing, samples and sample dilutions should be between pH 6-8). Tris buffer may be used to prepare samples for any of our endotoxin detection assays. Contains <0.005 EU/ml endotoxin.Storage: 2°C - 8°CContent:1 vial
Rockland Immunochemicals 10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2) - MB-075-1000
Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.
Boston Bioproducts Inc Paraformaldehyde (4%, PBS) - 250ml
Paraformaldehyde solution is a neutral fixative solution used to fix tissues and cells in immunohistochemistry and immunocytochemistry protocols. Stabilized in a phosphate buffer for delivery, this reagent actively cross-links proteins and DNA, immobilizing cellular components and maintaining the integrity of cell morphology. This reagent supports the accurate visualization of cells and tissues under a microscope, ensuring that researchers can observe fine details without degradation.
STEMCELL Technologies HBSS with 10 mM HEPES, Without Phenol Red, Size: 500 mL
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating, or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
ProteinSimple Maurice CE-SDS Running Buffer - Top, 12/pk
Pre-filled vial assembly containing top running buffer for CE-SDS application. For use with the cartridge insert (046-124) of the CE-SDS cartridge (PS-MC02-S) on Maurice and Maurice S. systems only. 12 Vials.
Bioworld Tris-HCl Buffer 10mM, pH 8.0, Sterile | 1185-53-1 | MFCD00012590 | 500mL
Tris-HCl Buffer 10mM, pH 8.0, Sterile | MW: 157.596 | 1185-53-1 | MFCD00012590 | 500mL
STEMCELL Technologies D-PBS (Without Ca++ and Mg++), Size: 500 mL
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Abcam Antigen Retrieval Buffer (100 x Tris-EDTA Buffer, pH 9.0), 125ML
Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
ProteinSimple Maurice CE-SDS Running Buffer - Bottom
Running buffer for CE-SDS application. 15 mL. For use with CE-SDS cartridge (PS-MC02-S) on Maurice (090-000) and Maurice S. (090-001) systems only.
Teknova 10mM Tris-HCl with 0.05% Tween-20, pH 8.0. 250mL, Sterile
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boca Scientific Inc Antibody Stabilizer PBS Base, 50ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.