Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
STEMCELL Technologies D-PBS (Without Ca++ and Mg++), Size: 500 mL
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 100mL
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
FUJIFILM Biosciences PBS 10X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 10X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration). Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.
Boston Bioproducts Inc Tris-Glycine-SDS Running Buffer 10X, 4 L, 8.50 ± 0.20 pH, 250 mM Tris, 1.92 M Glycine, 1% SDS, Anode and Cathode buffer, SDS-PAGE, Western blotting
This Tris-Glycine SDS Running Buffer (10X) is specifically formulated for use in protein electrophoresis applications, such as SDS-PAGE and Western blotting. It plays a vital role in the separation and resolution of denatured proteins. Upon dilution to the recommended working concentration, the buffer ensures consistent results with well-resolved protein bands. Furthermore, this buffer can function effectively as both the anode and cathode buffer, maintaining the pH essential for optimum electrophoresis performance.
- Volume: 4 L
- Concentration: 10X
- pH: 8.50 ± 0.20
- Application: SDS-PAGE, Western blotting
- Function: Separation of denatured proteins
- Recommended dilution: 10-fold with deionized water for 1X use
- Storage conditions: Room temperature
- Filtered through: 1-micron filter
- Quality control testing: Appearance, pH
Teknova 10X PBS Solution, pH 6.5 (at 1X). 1000 mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Charles River Laboratories 4 mL 0.5 M MgSO4, 1 M Tris buffer, 6/pk
4 mL 0.5 M MgSO4, 1 M Tris buffer, 6/pk.
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Cell Signaling Technology Tris Buffered Saline (TBS-10X) 1 liter
Tris Buffered Saline (TBS-10X) 1 liter