Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tricine-buffered Saline (5X) pH 8.0, 250 ml
Tricine-buffered Saline (5X) pH 8.0, 250 ml.
Bioworld Tris-Triton Buffer, 500 mL
Tris-Triton bufferis the idealbuffer with detergent required to release membrane- or cytoskeleton- bound proteins.Contains: 10mM Tris (pH 7.4), 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% Glycerol and 0.1% SDSAppearance: Liquid
Quality Biological Inc Tris HCl (2 M) pH 6.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
GENERAL LABORATORY PRODUCTS INC SALINE .85 TW80 PC20X125 100CS
NC3313467 SALINE .85 TW80 PC20X125 100CS
Medchemexpress LLC MES potassium | 39946-25-3 | 99.88% | 100 G
MES (2-Morpholinoethanesulphonic acid) potassium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer and is widely used in biochemistry and molecular biology experiments such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments (e.g., cell culture, enzyme activity determination, electrophoresis, protein studies)
- Zwitterionic buffer, provides effective buffering capacity around pH 6.15
- Pka value changes with temperature, requiring consideration for different temperatures
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions (e.g., Mg2+, Ca2+, Mn2+), suitable for studies involving metal ions
Medchemexpress LLC 4-Morpholineethanesulfonic acid, potassium salt | 39946-25-3 | 99.9% | 10 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. As a Good's buffer, it is utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15 and shows poor affinity with metal ions, making it suitable for studies involving them.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at around pH 6.15
- Poor affinity with metal ions, making it suitable for studies involving metal ions
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 500ml
HEPES solution | Mol Wt: 238.306 | 7365-45-9 | MFCD00006158 | 500ml
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g