Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 100G
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt 99.5 tit1KG
HEPES sodium salt 99.5 tit1KG
Research Products International Corp Bicine Ultra-Pure, 100 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659878.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 1 Kg
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is an effective biochemical reagent used to prepare buffer solutions for maintaining a consistent pH environment in laboratory experiments. This 1kg formulation is ideal for high-volume applications in protein chemistry, electrophoresis, and microbiology. PIPES buffers are widely used in studies requiring a neutral pH of 7.0 to stabilize proteins, enzymes, and cells. The disodium salt form provides a stronger buffering capacity and is often used in cell culture media, biochemical assays, and other research areas that demand precise pH control for accurate experimental results.
Medchemexpress LLC 2-methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.4% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptor agonist, with EC50s of 19, 6.2, and 5 nM for human P2Y13, mouse P2Y13 and human P2Y12, respectively. It has pEC50s of 8.29 and 5.75 for human P2Y1 and rat P2Y6, respectively. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptor agonist
- Acts on human P2Y13, mouse P2Y13, human P2Y12, human P2Y1 and rat P2Y6 receptors
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1
Alkali Scientific MES Buffer, Anhydrous, 100 G
MES Buffer, Anhydrous, 100g, is a highly effective biochemical reagent used for pH buffering in laboratory research. The anhydrous form of MES ensures that the buffer is stable and free from moisture, making it ideal for sensitive reactions where precise control of pH is necessary. MES is widely used in enzyme assays, protein purification, and cell culture applications, where it maintains a stable pH environment. The 100g volume is suitable for small-scale experiments, providing enough reagent for short-term studies or pilot projects. MES Buffer, Anhydrous, is an essential reagent for maintaining consistent pH in a variety of biochemical and biological applications.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration25KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659941.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 500g volume is a biochemical reagent used for pH buffering in various laboratory applications, particularly where enhanced ionic strength is required. This sodium salt form is ideal for experiments that demand robust pH control, such as in cell culture, enzyme assays, and protein purification. The 500g size is ideal for medium-scale laboratory use, providing enough reagent for multiple experiments or ongoing studies. MES Sodium Salt is non-interfering, making it suitable for sensitive biological systems and molecular biology studies where maintaining a stable pH is crucial for accurate results.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660290.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg
HEPES, Free Acid is a widely used biochemical reagent, available in a 25kg package for larger-scale applications. This highly stable buffer is effective in maintaining a pH range of 6.8–8.2, making it ideal for a variety of biochemical and biological applications such as protein analysis, cell culture, and drug formulation. HEPES helps maintain physiological pH levels, preventing changes that could interfere with experiments. This bulk package is especially beneficial for laboratories requiring frequent buffer preparation, allowing for efficient cost-saving and consistent results across a wide range of biological and chemical protocols.
Apexbio Technology LLC MES sodium salt 71119-23-8 1g
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Sigma Aldrich Fine Chemicals Biosciences PIPES Dipotassium Salt | 108321-27-3 | MFCD00069751 | 100g
PIPES Dipotassium Salt | Purity: 99% | Mol Wt: 378.55 | 108321-27-3 | MFCD00069751 | 100g
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 1kg
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 1kg