Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt | 76836-02-7 | MFCD00064349 | 100 g
PIPES disodium salt | Purity: ≥99% | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 100 g
Rockland Immunochemicals 10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20) - MB-013
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Rockland Immunochemicals 10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) - MB-008
Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
Rockland Immunochemicals TM Buffer (50 mM Tris Cl, pH 7.5, 10 mM Magnesium Sulfate) - MB-059
1X TM Buffer (50 mM Tris HCl, 10 mM Magnesium Sulfate at pH 7.5) is suitable for molecular biology assays.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt BioPerformance Certified, suitable for cell culture, >=99.5% | 75277-39-3 | MFCD00036463 | 500G
HEPES sodium salt BioPerformance Certified, suitable for cell culture, >=99.5% | Purity: >=99.5% | Mol Wt: 260.29 | 75277-39-3 | MFCD00036463 | 500G
Growcells PBS 20X 2L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Tris-Glycine-SDS PAGE Buffer (10X), Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration, 4 LT
![Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-A22500-100.0.JPG-250.jpg)
Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
![Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31020-100.0.JPG-250.jpg)
Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Bioworld MODIFIED DULBECCO S PBS PACKS
Modified Dulbecco\'s PBS Packs are pouches of dry-blend powder that are each sufficient to make 500mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Dry-Blend Packs of m...
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul50G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.