Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC Annexin V-HF647/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes 7-AAD specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot penetrate intact cell membranes However in late-apoptotic and dead cells membrane integrity is compromised allowing 7-AAD to enter and stain nuclear DNA Co-staining with Annexin V-HF647 and 7-AAD thus differentiates cells in various apoptotic stages and dead cells
Apexbio Technology LLC Annexin V-HF488/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes 7-AAD can specifically bind to double-stranded DNA resulting in red fluorescence Normally 7-AAD cannot pass through intact cell membranes however it can penetrate the cell membranes of late-stage apoptotic and dead cells staining their nuclei Combined staining with Annexin V-HF488 and 7-AAD allows differentiation of cells in various stages of apoptosis and dead cells
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (HF594) 100tests
EdU Flow Cytometry Assay Kits (HF594) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The EdU Flow Cytometry Assay Kits (HF594) use HyperFluor 594 azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (HyperFluor 594 azide excitation maximum at 590 nm emission maximum at 617 nm)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X without K, pH 7.4) - 1L
Phosphate Buffered Saline PBS (10X without K pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Bioworld PBS-Casein, 0.5%, 500 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 6X100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (6 x 100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 6 X 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Apexbio Technology LLC Annexin V-APC/SYTOX Green Apoptosis Kit 20tests
Annexin V-APC/SYTOX Green Apoptosis Kit is possible to differentiate cells at various stages of apoptosis and distinguish dead cells Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Moreover this kit contains SYTOX Green a nuclear probe that binds specifically to double-stranded DNA emitting green fluorescence SYTOX Green normally cannot permeate intact cell membranes It can penetrate the membranes of late-stage apoptotic cells or dead cells thus staining their nuclei
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cell crude extracts contain various endogenous enzymes such as phosphatases and proteases capable of degrading proteins present in the extracts The optimal strategy to enhance intact protein yield is adding specific inhibitors targeting these enzymes This Protease Inhibitor Cocktail is used in fungal and yeast extracts to improve protein stability by inhibiting proteases responsible for degrading both phosphorylated and non-phosphorylated protein substrates This Protease Inhibitor Cocktail consists of individual compounds including AEBSF 1 10-Phenanthroline E-64 and Pepstatin A which provide broad-spectrum specific inhibition of cysteine proteases serine proteases aspartic proteases and metalloproteases It is optimized and tested for fungal and yeast extracts This cocktail is available as a ready-to-use solution dissolved in DMSO
Apexbio Technology LLC Annexin V-Cy3/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but flips from the inner to the outer leaflet during the early stages of apoptosis becoming exposed to the extracellular environment Annexin V has a high affinity for PS By conjugating Annexin V with Cy3 the labeled Annexin V serves as a fluorescent probe enabling detection of apoptosis events via flow cytometry or fluorescence microscopy within only 10 20 minutes staining SYTOX Green is a membrane-impermeable cyanine dye that normally cannot penetrate intact cell membranes However during late-stage apoptosis or necrosis SYTOX Green can cross disrupted regions of the cell membrane reach the nucleus and bind DNA double helix molecules forming SYTOX Green-DNA complexes Binding increases fluorescence intensity over 500-fold generating bright green fluorescence Thus SYTOX Green stains o
Teknova 10X PBS Solution. 1000mL, Sterile. 2 pack.
1.35M Sodium Chloride,27mM Potassium Chloride,43mM Disodium Phosphate,14mM Monopotassium Phosphate
Dojindo Molecular Technologies Inc TES (100G), Good's buffer, CAS: 7365-44-8, pka=7.5, Active pH 6.8-8.2, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.