Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
American Research Products Inc TRIS-ACETATE BUFFER, 0.2M,
TRIS-Acetate Buffer, 0.2M, pH 7.8-500ml. 0.2M Tris base dissolved in 18.2 megohms water, pH adjusted to 7.8 with glacial acetic acid and filtered through 0.22-micron filter.
Biotang Inc Tricine-buffered Saline (5X) pH 7.5, 250 ml
Tricine-buffered Saline (5X) pH 7.5, 250 ml.
Biotang Inc Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
![eMolecules 1629128-52-4 | benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | Pharmablock | MFCD18071452 | 275.348 | C16H21NO3 | 97.000 | OC[C@H]1C[C@@H]2CC[C@H](C1)N2C(=O)OCc1ccccc1 | 1g | 551164315](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502156986.JPG-250.jpg)
eMolecules 1629128-52-4 | benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | Pharmablock | MFCD18071452 | 275.348 | C16H21NO3 | 97.000 | OC[C@H]1C[C@@H]2CC[C@H](C1)N2C(=O)OCc1ccccc1 | 1g | 551164315
benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | Pharmablock | 1629128-52-4 | MFCD18071452 | 275.348 | C16H21NO3 | 97.000 | OC[C@H]1C[C@@H]2CC[C@H](C1)N2C(=O)OCc1ccccc1 | 1g | 551164315
Bioworld TBS Buffer, High Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is high salt used for Western blot washing with a concentration of 10X. It contains 0.5M Tris, 4.5M NaCl and has a pH level of 7.4. Buffer is stored at room temperature.TBS is isotonic and non-toxic, and is used to dilute substances. It is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Bioworld TTE Buffer, Ready-Pack (20X), 1 PK(s)
20x TTE (Tris-TAPS-EDTA) buffer pack can be dissolved into 2L of water to make 2L of 1x TTE solution.
Bioworld TBE Sample Buffer (4X), 25 mL
Tris-Borate Buffer, EDTA (TBE) Sample buffer for nucleic acid analysis. Technical Information: 4X concentration Tris base (270mM) Boric Acid (270mM) Glycerol (36%) Bromophenol blue G (0.025%) Xylene Cyanol (0.025%) EDTA (6mM)
CHEMSCIENCE Soluble Sulfate for SO3
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Kd Medical Inc TRIS GLYC SDS BUFFER, 10X, 4L
10X Tris Glycine SDS is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. One 4 liter cubitainer.
Teknova 1M Tris-HCl, pH 8.0 Solution. 4L, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.