Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture | 1132-61-2 | MFCD00006183 | 100g
MOPS BioPerformance Certified, suitable for cell culture | Purity: 99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100g
Research Products International Corp MOPS Free Acid 50 G
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes.
Heavy Metals: <5 ppm
pKa: (20°C) 7.20 ± 0.2
pH Range: 6.5-7.9
Research Products International Corp Bicine Ultra-Pure, 250 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Medchemexpress LLC Pipes dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 100 G
PIPES (dipotassium) is the dipotassium salt of the zwitterionic buffering agent PIPES, provided as a white to off-white solid. It offers stable buffering near physiological pH with low metal ion binding, making it suitable for biochemical and cell biology applications such as assay buffers, electrophoresis, and sample preparation.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Bioworld TAE Buffer, Quick-Mix Pack (25X), 4 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules