Phen Green SK diacetate (5/6-mixture) is a metal ion-sensitive fluorescent probe that can penetrate cell membranes. It reacts with various metal ions like Fe2+, Cd2+, Co2+, Ni2+, and Zn2+. When Phen Green SK diacetate chelates Fe2+, it causes fluorescence quenching, which can be reversed by adding a membrane-permeable chelator, reflecting changes in the intracellular chelatable iron pool.
- Metal ion-sensitive fluorescent probe.
- Can penetrate cell membranes.
- Reacts with a variety of metal ions including Fe2+, Cd2+, Co2+, Ni2+, and Zn2+.
- Chelates Fe2+, leading to fluorescence quenching that can be restored, indicating changes in the intracellular chelatable iron pool.
- Excitation/emission maxima are 507/532 nm.
- Used to detect intracellular Fe2+ levels.
- Can be used with a lipid peroxidation indicator dye to assess the ferroptosis state of cells.