Animal-Free M-CSF Protein, Human (His) is a recombinant human macrophage colony-stimulating factor produced in E. coli and supplied lyophilized with a C-terminal 6xHis tag for research and cell-culture applications. It regulates macrophage survival, differentiation, and function, and is characterized for biological activity and high purity.
- Lyophilized C-terminal 6xHis-tagged recombinant human M-CSF.
- Supplied as a 50 μg vial for research use.
- Produced in E. coli using animal-free processes.
- Predicted molecular mass 13.3 kDa; ~13-26 kDa by reducing SDS-PAGE.
- Purity >95% as determined by reducing SDS-PAGE.
- Specific activity >2.5 x 10^8 IU/mg; ED50 <1 ng/mL (NFS-60 assay).
- Formulated in PBS with trehalose; reconstitute to 100-200 μg/mL in ddH2O.
- Store at -20°C; freeze aliquots at -20°C or -80°C for long-term storage.