HPLC Columns and Cartridges
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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients
Thermo Scientific™ Accucore™ C18 HPLC Columns
Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.
| Pore Size | 80 Å |
|---|---|
| Particle Size | 2.6 μm |
| Carbon Load | 9% |
| Stationary Phase | C18 |
| Temperature | 70°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| pH | 1 to 11 |
| Surface Area | 130 m²/g |
| Particle Shape | Solid Core |
| USP Type | L1 |
Thermo Scientific™ DNAPac™ RP Columns
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.
Thermo Scientific™ ProPac™ WCX-10 HPLC Columns
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution.
| Pore Size | Nonporous |
|---|---|
| Particle Size | 10 μm |
| Stationary Phase | Carboxylate |
| Temperature | 60°C |
| Phase | Ion Exchange |
| Column Type | Ion Exchange |
| pH | 2 to 12 |
| Quantity | Each |
| Product Line | ProPac |
Thermo Scientific™ MAbPac™ Reversed Phase HPLC Columns
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Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins.
| Pore Size | 1500 Å |
|---|---|
| Particle Size | 4 μm |
| Phase | Reversed Phase |
| Max. Pressure | 4000 psi (275 bar) |
TSKgel™ G3000SWXL HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel™ SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
Thermo Scientific™ Aquasil™ 3μm C18 HPLC Columns
Available in limited dimensions. Please contact Customer Service for more details.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 3 μm |
| Carbon Load | 12% |
| Stationary Phase | C18 |
| Phase | Reversed Phase |
| For Use With | LC/MS |
| Endcapped | Yes |
| Packing Material | Silica |
| Column Type | Reversed Phase |
| pH | 2 to 8 |
| Surface Area | 310 m²/g |
| USP Type | L1 |
| Column Format | Analytical Column |
Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.
Thermo Scientific™ MAbPac™ SEC-1 Size Exclusion Chromatography HPLC Columns
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Use these SEC columns for monoclonal antibody (mAb) analysis, including the high-resolution separation of monomers, aggregates and fragments.
| Pore Size | 300 Å |
|---|---|
| Particle Size | 5 μm |
| Solvents | 100% Organic Solvents |
| Temperature | 30°C |
| Phase | Size Exclusion |
| Column Type | Size Exclusion |
| pH | 2.5 to 7.5 |
| Quantity | Each |
| Product Line | MAbPac |
MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 5 μm Particle Size
The bare silica stationary phase of Ascentis™ Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Silica |
| Phase | Reversed Phase |
| Matrix | Silica Phase/Fused-Core Particle Platform |
| Endcapped | No |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å AQ-C18 HPLC Column, 2.7 μm Particle Size
A high-speed, high-performance liquid chromatography column based on the Fused-Core™ particle design.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Carbon Load | 6.7% |
| Phase | Reversed Phase |
| Matrix | AQ-C18 Phase/Fused-Core™ Silica Particle Platform |
| Endcapped | Yes |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Surface Area | 135 m2/g |
| Product Line | Ascentis |
| USP Type | L1 |
| Pore Size | 120 Å |
|---|---|
| Particle Size | 2.7 μm |
| Phase | Reversed Phase |
| Product Type | HPLC Column |
| Diameter (Metric) | 1.5 mm |
| Material | Stainless Steel |
| Technique | HPLC |
MilliporeSigma™ Supelco™ Ascentis™ Express C18 HPLC Column, 2.7 μm Particle Size
Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.
| Pore Size | 90 Å |
|---|---|
| Stationary Phase | C18 (octadecyl) |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Material | Stainless Steel |
| Max. Pressure | 600 bar(9000 psi) |
| Column Format | Analytical Column |
| Particle Size | 2.7 μm |
| Matrix | C18 (octadecyl) Phase/Fused-Core Particle Platform |
| Product Type | HPLC/UHPLC Column |
| Packing Material | Fused-Core particle platform superficially porous particle |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
| USP Type | USP L1 |
TSKgel™ ODS-100Z HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ ODS-100Z column's bonded phases are prepared by reacting the silica surface with a difunctional octadecylsilane reagent, followed by repeated endcapping with a monofunctional trimethylsilane reagent.
| Particle Size | 3 μm |
|---|---|
| Solvents | 0 to 100% organic solvent |
| Carbon Load | 20% |
| Stationary Phase | C18 (octadecyl) |
| Temperature | 10°C to 50°C |
| Phase | Reversed Phase |
| Matrix | Spherical Silica |
| pH | 2.0 - 7.5 |
| Material | Stainless Steel |
| Surface Area | 450 m2/g |
| Product Line | TSKgel |
| USP Type | L1 |
MilliporeSigma™ Supelco™ Ascentis™ Si HPLC Column, 3 μm Particle Size
The Ascentis™ Si is a high loading capacity silica with excellent peak shape. The Ascentis™ Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | SIlica |
| Phase | Reversed Phase |
| Matrix | Silica Gel High Purity, Spherical/Silica Phase |
| Endcapped | No |
| pH | 2 to 6 |
| Technique | Fused-Core HPLC |
| Surface Area | 450 m2/g |
| Product Line | Ascentis |