Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,601)

Singleplex and Multiplex Bead Based Immunoassays (6,181)

ELISA Substrates and Reagents (2,340)

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R&D Systems™ Human Total IL-18/IL-1F4 Quantikine ELISA Kit

Human Total IL-18/IL-1F4 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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R&D Systems™ Human IL-8/CXCL8 Quantikine ELISA Kit

Human IL-8/CXCL8 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human IL-8
Target Species	Human
Conjugate	HRP
Gene ID (Entrez)	3576
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Synonym	3-10 C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP1, GCP-1 TSG-1, IL8, interleukin 8, K60, LAI, LECT, LUCT, LYNAP, MDNCF, MDNCFb-ENAP, member 8, MONAP, MONAPGCP1, NAF, NAP1, NAP-1 NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10 C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1
Assay Sensitivity	7.5pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	31.20 - 2000pg/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit

Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Assay Activity Kit
Content And Storage	96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Target Kit Named	Superoxide Dismutase (SOD)

Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

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Invitrogen™ Human Amyloid beta 42 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	4.3%
Intraassay CV	3.6%
Incubation Time	4 hr.
Gene Symbol	APP
Total Assay Time	4 hr
Sample Volume	Cerebrospinal Fluid 50 μL, Supernatant 50 μL, Tissue Homogenate 50 μL
Assay Sensitivity	<10 pg/mL
Storage Requirements	2-8°C
Concentration	15.6 to 1000 pg/mL
Assay Range	15.6-1000 pg/mL
Marker Gene	Aβ 42
Test Time	1 hr 20 min
Accession Number	P05067
Regulatory Status	RUO
Target	Amyloid beta 42
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-IgG HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Detection Antibody,Anti-IgG HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	351
Sample Type	Cerebrospinal Fluid,Supernatant,Tissue Homogenates
Label or Dye	Biotin

Invitrogen™ Mouse IFN gamma ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Gene Symbol	IFNG
Total Assay Time	3 hr 10 min
Assay Sensitivity	1.7 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
Test Time	1 hr 20 min
Accession Number	P01580
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Gene Alias	BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-gBoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g;
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	15978
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human MUC5AC ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	MUC5AC
Total Assay Time	3 hr 30 min
Assay Sensitivity	0.19 ng/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	0.31-20 ng/mL
Test Time	1 hr 20 min
Accession Number	P98088
Regulatory Status	RUO
Target	MUC5AC
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	2210005L13Rik; gastric mucin; LeB; lewis B blood group antigen; LOW QUALITY PROTEIN: mucin-5AC; major airway glycoprotein; MGM; MUC5; MUC5AC; MUC-5AC; mucin; mucin 5, subtypes A and C; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5ac; mucin 5AC, oligomeric mucus/gel-forming; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin-5 subtype AC, tracheobronchial; Mucin-5AC; TBM; Tracheobronchial mucin2210005L13Rik; gastric mucin; LeB; lewis B blood group antigen; LOW QUALITY PROTEIN: mucin-5AC; major airway glycoprotein; MGM; MUC5; MUC5AC; MUC-5AC; mucin; mucin 5, subtypes A and C; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5ac; mucin 5AC, oligomeric mucus/gel-forming; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin-5 subtype AC, tracheobronchial; Mucin-5AC; TBM; Tracheobronchial mucin;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	4586
Sample Type	Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Mouse SAA ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	5.6%
Intraassay CV	4.4%
Gene Symbol	Saa, SAA1
Total Assay Time	1 hr 15 min
Assay Sensitivity	0.03 μg/mL
Storage Requirements	2-8°C
Assay Range	0.062-0.5 μg/mL
Test Time	1 hr 20 min
Accession Number	P05366
Regulatory Status	RUO
Target	SAA
Kit Contents	Pre-coated 96 well plate, Standard/Control, Standard Dilution Buffer, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution,Standard/Control,Standard Dilution Buffer,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution
Gene Alias	amyloid a; Amyloid fibril protein AA; Amyloid protein A; MGC111216; PIG4; SAA; SAA1; Saa-1; Saa2; serum amyloid A; serum amyloid A 1; serum amyloid A 2; serum amyloid A protein; serum amyloid A1; Serum amyloid A1 protein; Serum amyloid A-1 protein; serum amyloid A-like protein; Serum amyloid protein A(2-102); Serum amyloid protein A(2-103); Serum amyloid protein A(2-104); Serum amyloid protein A(3-104); Serum amyloid protein A(4-101); Serum Amyloid-A; TP53I4; tumor protein p53 inducible protein 4amyloid a; Amyloid fibril protein AA; Amyloid protein A; MGC111216; PIG4; SAA; SAA1; Saa-1; Saa2; serum amyloid A; serum amyloid A 1; serum amyloid A 2; serum amyloid A protein; serum amyloid A1; Serum amyloid A1 protein; Serum amyloid A-1 protein; serum amyloid A-like protein; Serum amyloid protein A(2-102); Serum amyloid protein A(2-103); Serum amyloid protein A(2-104); Serum amyloid protein A(3-104); Serum amyloid protein A(4-101); Serum Amyloid-A; TP53I4; tumor protein p53 inducible protein 4; Saa-1; Saa2
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	111345, 20208
Sample Type	Plasma,Serum
Label or Dye	HRP

Invitrogen™ Human hCG-beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	CGB3
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	40 pg/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	40-10000 pg/mL
Test Time	1 hr 20 min
Accession Number	P0DN86
Regulatory Status	RUO
Target	hCG beta
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	1082
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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R&D Systems™ Human IFN-gamma Quantikine ELISA Kit

solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma.

Pricing & Availability
Specifications

Target	IFN-gamma
Specificity	Natural and Recombinant Human IFN-gamma
Target Species	Human
Gene ID (Entrez)	3458
Sample Type	Cell Culture Supernatant,Plasma (EDTA),Heparin Plasma,Serum
Synonym	IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
Assay Sensitivity	5.69 pg/mL
Storage Requirements	Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL
For Use With (Application)	ELISA

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R&D Systems™ Human IFN-gamma DuoSet ELISA

Human IFN-gamma DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Immunoassay Kit Format	96-well Plate
Target	Interferon gamma
Kit Contents	96 well Micro Plates,PBS,Plate Sealers,Reagent Diluents Concentrate 2,Stop Solution,Substrate Solution,Wash Buffer
Target Species	Human
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	9.38 - 600pg/mL

Invitrogen™ Mouse Estradiol Rapid ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Total Assay Time	1 hr 30 min
Assay Sensitivity	1.88 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	3.13-200 pg/mL
Test Time	15 min
Regulatory Status	RUO
Target	Estradiol
Kit Contents	Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,HRP Conjugate,Standard & Sample Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	17-Beta-Estradiol; 17-beta-HSD17-Beta-Estradiol; 17-beta-HSD;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Saliva,Serum,Urine
Label or Dye	HRP

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R&D Systems™ Mouse IL-10 Quantikine ELISA Kit

Mouse IL-10 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Mouse IL-10
Target Species	Mouse
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	16153
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum
Synonym	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

Invitrogen™ Human IFN gamma ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	FAM
Gene Symbol	IFNG
Total Assay Time	2 hr
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.01 pg/mL
Storage Requirements	-20°C
Assay Range	0.0128-5000 pg/mL
For Use With (Application)	Cytokine Protein Quantitation
Test Time	30 min
Detection Method	Taqman™ Real-time PCR
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

Immunoassay Reagents and Kits

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R&D Systems™ Human Total IL-18/IL-1F4 Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human IL-8/CXCL8 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human IFN-gamma Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human IFN-gamma DuoSet ELISA GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Mouse IL-10 Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Invitrogen™ Human IFN gamma ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

R&D Systems™ Human Total IL-18/IL-1F4 Quantikine ELISA Kit

R&D Systems™ Human IL-8/CXCL8 Quantikine ELISA Kit

R&D Systems™ Human IFN-gamma Quantikine ELISA Kit

R&D Systems™ Human IFN-gamma DuoSet ELISA

R&D Systems™ Mouse IL-10 Quantikine ELISA Kit

Invitrogen™ Human IFN gamma ProQuantum Immunoassay Kit