Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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R&D Systems™ Human VEGF Quantikine ELISA Kit
Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b. |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene ID (Entrez) | 7422 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum |
| Sample Volume | Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL) |
| Synonym | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor |
| Assay Sensitivity | 5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
| For Use With (Application) | ELISA |
| Cross Reactivity | Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules. |
Invitrogen™ Human IFN gamma ELISA Kit, High Sensitivity
ELISA
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 3458 |
| Interassay CV | 7.1% |
| Intraassay CV | 6.8 |
| Assay Sensitivity | 0.06 pg/mL |
| Assay Range | 0.16 to 10.0 pg/mL |
R&D Systems™ Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Mouse IL-10 |
|---|---|
| Target Species | Mouse |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 16153 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum |
| Synonym | CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Novus Biologicals™ Mouse Amylin ELISA Kit (Colorimetric)
Mouse Amylin ELISA Kit (Colorimetric) from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit
Human IL-12 p70 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human IL-12p70 |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3592 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum,Urine |
| Sample Volume | Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL),Urine (200uL) |
| Synonym | CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12 A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35 |
| Assay Sensitivity | 5pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 7.80 - 500pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Invitrogen™ Triglyceride (TG) Colorimetric Assay Kit
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
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Invitrogen™ Human Glucagon ELISA Kit
ELISA
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| Protein Family | GPCR (G-Protein Coupled Receptors) |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 2.5 pg/mL |
| Research Discipline | Signal Transduction |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | Glucagon |
| Specificity | This ELISA pair antibody detects human Glucagon. Other species not determined. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 2.5 pg/mL to 130 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | Gcg |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 2.5-130 pg/mL |
| Accession Number | P01275 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 2641 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | GLP1, GLP2, GRPP |
Invitrogen™ Human Growth Hormone ELISA Kit
ELISA
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| Protein Family | Growth Factors & Receptors |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 25 μL, Serum 25 μL, Supernatant 100 μL |
| Assay Sensitivity | 4 pg/mL |
| Research Discipline | Biomarker-Related Pathways |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | Growth Hormone |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 4 pg/mL to 600 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | Gh1 |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 4-600 pg/mL |
| Accession Number | P01241 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 2688 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | GH, GH-N, GHN, IGHD1B, hGH-N |
Novus Biologicals™ Human IgA1 ELISA Kit (Colorimetric)
Human IgA1 ELISA Kit (Colorimetric) measures IgA1 in Serum, plasma, cell supernatant, and other biological fluids.
| Assay Sensitivity | 0.94 ng/mL |
|---|---|
| Assay Range | 1.56 to 100 ng/mL |
Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit
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ELISA
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| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hr |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.3 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.32-25000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 min |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | Q16552 |
| Regulatory Status | RUO |
| Target | IL-17A |
| Kit Contents | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 3605 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
R&D Systems™ Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Target | TIM-1/KIM-1/HAVCR |
|---|---|
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 26762 |
| Sample Type | Urine |
| Gene Symbol | HAVCR1 |
| Assay Sensitivity | 0.046 ng/mL |
| Storage Requirements | Store the unopened product at 2°C to 8°C. Do not use past expiration date. |
| Assay Range | 0.16 to 10 ng/mL |
| For Use With (Application) | ELISA |
Invitrogen™ Human RANKL (Soluble) ELISA Kit
ELISA
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Gene Symbol | TNFSF11 |
| Total Assay Time | 3 hr 30 min |
| Assay Sensitivity | 9.38 pg/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 15.63-1000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | O14788 |
| Regulatory Status | RUO |
| Target | RANKL (RANK Ligand) |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer |
| Gene Alias | CD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokineCD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokine; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 8600 |
| Sample Type | Plasma,Serum |
| Label or Dye | Biotin |
Invitrogen™ Monkey Complement C5a ELISA Kit
ELISA
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| Shipping Condition | Wet Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Monkey |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Total Assay Time | 3 hr 30 min |
| Assay Sensitivity | 0.09 ng/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 0.16-10 ng/mL |
| Test Time | 1 hr 20 min |
| Regulatory Status | RUO |
| Target | Complement C5a |
| Kit Contents | Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis,Reference Standard,Concentrated Biotinylated Detection Ab(100x),Concentrated HRP Conjugate (100x),Reference Standard & Sample Diluent,Biotinylated Detection Ab Diluent,HRP Conjugate Diluent,Concentrated Wash Buffer(25x),Substrate Reagent,Stop Solution,Plate Sealer,Product Description,Certificate of Analysis |
| Gene Alias | anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C5; C5a; C5a anaphylatoxin; C5b; C5D; CH; CO4; complement C5; Complement C5 alpha chain; Complement C5 alpha' chain; Complement C5 beta chain; complement component 5; CPAMD3; CPAMD4; DADB-112B14.11; ECLZB; FLJ17816; FLJ17822; FLJ60561; Hc; He; Hemolytic complement; MGC142298; MGC164979; prepro-C5; RGD1561905anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C5; C5a; C5a anaphylatoxin; C5b; C5D; CH; CO4; complement C5; Complement C5 alpha chain; Complement C5 alpha' chain; Complement C5 beta chain; complement component 5; CPAMD3; CPAMD4; DADB-112B14.11; ECLZB; FLJ17816; FLJ17822; FLJ60561; Hc; He; Hemolytic complement; MGC142298; MGC164979; prepro-C5; RGD1561905; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Sample Type | Plasma,Serum |
| Label or Dye | Biotin |