Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Mouse Adiponectin ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Mouse IL-4 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10% |
| Intraassay CV | 5% |
| Gene Symbol | Il4 |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2.0 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 3.9-250 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P07750 |
| Regulatory Status | RUO |
| Target | IL-4 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16189 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | B-cell stimulatory factor 1, B cell growth factor 1 |
Invitrogen™ Mouse IL-18 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 2.2% |
| Intraassay CV | 2.2 |
| Gene Symbol | Igif,Il-18 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 19.0 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 31.3 to 2,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P70380 |
| Regulatory Status | RUO |
| Target | IL-18 |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16173 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interleukin-18, Interferon-gamma-inducing factor |
Invitrogen™ Mouse Granzyme B Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Human BDNF ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | BDNF |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 80 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.066 to 16 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF |
| Regulatory Status | RUO |
| Target | BDNF |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 627 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human IgE Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL |
| Assay Sensitivity | 7.8 ng/mL |
| Assay Range | 7.8 to 500 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Human IgE |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Human IgE protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3497 |
| Sample Type | Plasma,Serum |
| Synonym | Immunoglobulin E |
Invitrogen™ Human Tryptase beta-2/TPSB2 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Tpsb2 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 2 to 500 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | AV011504; EC 3.4.21; EC 3.4.21.59; mast cell protease 6; mast cell tryptase beta II; mast cell tryptase beta III; Mcp6; Mcp-6; Mcpt6; MMCP-6; rMCP-6; testicular tissue protein Li 163; TPS2; TPS2tryptase beta-2; Tpsb2; tryptase beta 2; tryptase beta 2 (gene/pseudogene); tryptase beta II; tryptase beta III; tryptase beta-2; Tryptase II; tryptase III; Tryptase-2; tryptaseB; tryptaseC; Tryptase-C |
| Regulatory Status | RUO |
| Target | TPSB2 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 64499 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human MMP-12 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Mmp12 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.04 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.04 to 10 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 3.4.24.65; AV378681; HME; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; Mme; Mmel; MMP; MMP12; MMP-12; MMPs |
| Regulatory Status | RUO |
| Target | MMP12 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4321 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human IL-10 Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Gene Symbol | BCGF-1,BCGF1,BSF-1,BSF1,IL-4 |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 2 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 2 to 300 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Accession Number | P22301 |
| Regulatory Status | RUO |
| Target | IL-10 |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3586 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interleukin-10 |
Invitrogen™ Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | S100A8, S100A9 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 35 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 35 to 8,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; Caga; Cagb; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK |
| Regulatory Status | RUO |
| Target | Calprotectin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 6279, 6280 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human SARS-CoV-2 RBD ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Assay Sensitivity | 4.5 pg/mL |
| Assay Range | 6.14 to 1500 pg/mL |
Invitrogen™ Human Endothelin-1 (ET-1) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Description | Endothelin-1 (ET-1) ELISA Kit |
| Interassay CV | 3.7% |
| Intraassay CV | 3.3 |
| Gene Symbol | EDN1 |
| Total Assay Time | 2 hrs 30 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 2.5 μL, Urine 50 μL |
| Assay Sensitivity | 0.579 pg/mL |
| Storage Requirements | -20°C |
| Research Discipline | Cell Signaling |
| Assay Range | 0.781 to 100 pg/mL |
| For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
| Test Time | 1 hrs 20 mins |
| Detection Method | Fluorescence |
| Accession Number | P05305 |
| Regulatory Status | RUO |
| Target | Endothelin 1 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers,Extraction Solution |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 1906 |
| Sample Type | Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
Invitrogen™ Human MMP9 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10.2% |
| Intraassay CV | 7.3 |
| Gene Symbol | CLG4B,GELB,MANDP2,MMP-9 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 0.05 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.23 to 15.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P14780 |
| Regulatory Status | RUO |
| Target | MMP-9 |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4318 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | 92 kDa gelatinase, Gelatinase B, GELB |
Invitrogen™ Mouse IL-12 p70 Uncoated ELISA Kit with Plates
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse,Mouse |
|---|---|
| Conjugate | HRP |
| Gene Symbol | IL12A, Il12b |
| Total Assay Time | 24 hr 30 min |
| Sample Volume | Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 15 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 15-2000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 45 min |
| Accession Number | P43431, P43432 |
| Regulatory Status | RUO |
| Target | IL-12 p70 |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16159, 16160 |
| Sample Type | Serum,Supernatant,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Interleukin-12, IL12, IL23 |
Invitrogen™ Human IL-21 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.7% |
| Intraassay CV | 5.8 |
| Gene Symbol | GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1 |
| Total Assay Time | 4 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2.0 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 78 to 5,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P10145 |
| Regulatory Status | RUO |
| Target | IL-21 |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 59067 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Protein 3-10 C, CXCL8, NAP-1, GCP-1 |