Protein Method Blocking Buffers
LICOR Intercept T20 (TBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Abcam Capsazepine, TRPV1 antagonist, 10MG
MW 376.9 Da, Purity >98%. TRPV1 vanilloid receptor antagonist (IC₅₀ = 562 nM). Analgesic in vivo.
The product is subject to the following: Abcam Restricted Use Statement
ILEX LIFE SCIENCES LLC Block Ace Powder: blocking reagent for immunological experiments (20 x 4 g)
Block Ace Powder: blocking reagent for immunological experiments. Block Ace is a blocking agent prepared from bovine milk proteins (lactoprotein). Since lactoprotein contains a lot of hydrophobic groups, it shows a high affinity to plastic, membrane filter, etc. Block Ace exhibits a superior blocking effect on nonspecific adsorption of antigens or antibodies in ELISA, RIA and immunoblocking methods compared with conventional blocking agents such as BSA (Bovine Serum Albumin). FEATURES: Since Block Ace has high blocking effects and maintains the background at a low level, it is useful for low-concentration measurement such as ELISA. Block Ave is heat-treated and thus bovine-derived antibodies, enzymes and viruses are inactivated. Block Ace is stable for heat and freezing. It can stabilize physiologically active substances and can be used as a diluting agent. Block Ace can be rapidly dissolved in water. MANUFACTURER: KAC Co., Ltd., Kyoto-city 603-8432, Japan.
Rockland Immunochemicals BlockOut (2X) - Universal Blocking Buffer for Western Blotting - MB-073-050
BlockOut is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with chemiluminiscent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Seracare Life Sciences Inc 10X TBS W/ 10% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 10% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. This formulation of TBST has a higher concentration of Tween 20 and provides a more stringent wash than standard TBST formulations.
Abcam He x amethonium bromide, nicotinic receptor blocker, 100MG
MW 362.19 Da. Ganglionic blocker; antagonist at α₃-containing nicotinic acetylcholine receptors (autonomic ganglia).
The product is subject to the following: Abcam Restricted Use Statement
Biocare Medical, LLC intelliPATH FLX Peroxidase Blocking Reagent - 20 ml
Peroxidazed Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations.
Boca Scientific Inc SmartBlock™, 500ml
SmartBlock™ is a BSA-free, peptide-based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces, removing background and preventing unwanted binding. This reagent was produced by chemically modifying peptides in a specific process that resulted in a peptide solution with strong blocking characteristics. SmartBlock™ has high lot-to-lot consistency that had previously only been obtained by synthetic blockers. For many assays, SmartBlock™ is an economical and reliable alternative to BSA-containing solutions. Problems caused by BSA within blockers, such as BSA-interactions and cross reactivities, can be eliminated by using SmartBlock™.
SCANTIBODIES LABORATORY INC HBR PLUS (3KC545) 1.0G
The 3KC545 Heterophilic Blocking Reagent Plus from Scantibodies Laboratory, Inc. was developed as an alternative for HBR-1. This product is compounded with immunoglobulins with different characteristics. Therefore, in addition to its active blocking characteristics, the special formulation and production procedures enhance its efficacy in its passive blocking ability as well. It has a concentration of 10.0 +/- 1.0 mg/ml.
Sigma Aldrich Fine Chemicals Biosciences Gelatin blocking buffer for Western blotting, powder blend | 1L
Gelatin blocking buffer for Western blotting, powder blend | 1L
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Phosphate Buffered Saline with Tween-20 (PBST) is a specialized variant of PBS, supplemented with Tween-20, a non-ionic surfactant. Tween-20 minimizes the non-specific binding and background noise, resuting in enhanced signal to noise ratio. PBST is often used in immunoassays such as ELISA and Western Blotting, where it serves as a washing and blocking buffer. With a stable buffering pH range between 7.0 and 7.4, PBST maintains ionic strength and protein solubility for biological assays.
Santa Cruz Biotechnology UltraCruz Blocking Reagent, 100 mL
Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications. Animal-free alternative to sera, BSA or non-fat dry milk. Designed to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applications. The UltraCruz Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
Biotang Inc GELATIN- Blocking Buffer in PBS1% GELATIN, 500 ml
GELATIN- Blocking Buffer in PBS1% GELATIN, 500 ml.
Biotang Inc BLOTTO IN PBS 250 ML, IBB-141. 5% nonfat dry milk in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate, 10 mM KCl, 137 mM NaCl), pH 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO IN PBS 250 ML, IBB-141. 5% nonfat dry milk in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate, 10 mM KCl, 137 mM NaCl), pH 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.