Protein Method Blocking Buffers
LI-COR Intercept (PBS) Blocking Buffer (25 x 500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; Excellent choice for biotin-streptavidin detection; Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays.Formulation: Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes. Shake well before each use.
Bioworld TBS/Casein, 100 mL
All blocks and dlluents are supplied in a 10X concentration of physiological saline buffer. pH range: 7.6 +/- 0.1
Teknova 10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile. 8 pack.
10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile. 8 pack.
LI-COR Intercept (PBS) Blocking Buffer and Diluent Kit
Try Intercept (PBS) Blocking Buffer and Intercept T20 (PBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation.Kit Components: Intercept (PBS) Blocking Buffer, 1 x 500 mL; Intercept T20 (PBS) Antibody Diluent, 2 x 500 mLShake well before each use.Intercept (PBS) Blocking Buffer and Intercept T20 (PBS) Antibody Diluent can be used for many immunoassays and applications, including: Quantitative Western Blot, Chemiluminescent Western Blot, In-Cell Western™ Assay, In-Gel Western, Protein Array, Glycoprotein Detection
IBA LifeSciences Biotin Blocking Buffer; 2 ml
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins such as the biotin carboxyl carrier protein (BCCP, 22 kDa) of E. coli. Biotin Blocking Buffer is recommended for blocking such biotinylated proteins on western blots prior to detection of Strep-tagII or Twin-Strep-tag fusion proteins with Strep-Tactin or Strep-TactinXT conjugates to prevent the detection of false positive bands. The buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins. Please note that usage of BioLock is not necessary when using StrepMAB-Classic, since this antibody does not bind to biotinylated proteins.