General Western Blot Reagents
LICORbio CellTag™ 520 Stain for In-Cell Western™ Assays (2 X 50 nmol)
CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 520 Stain is detected in the 520 nm channel. CellTag 520 Stain is applied to the cells during incubation with an IRDye Secondary Antibodies (e.g., IRDye 680RD or IRDye 800CW), and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
Biotium Dry Milk Powder, 4 x 25 g
Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Bioworld Nylon Membrane Stripping Buffer (10X), 500 mL
Product DescriptionNylon Membrane Stripping Buffer (10X) is a liquid used for the hybridization of probes to nucleic acids. Buffer contains Tris HCL at pH 8.0 (1mM), EDTA (100mM), and Denhardt’s solution (1X). To use this product, dilute at 10-fold with sterile deionized water and strip the membrane by 2 hours of incubation at 75°C.
Bioworld Nitrocellulose Stripping Buffer (10X), 250 mL
Product DescriptionNitrocellulose stripping buffer (10X) is used for hybridization of probes to nucleic acids. It is ideal for removal of secondary antibody after detection of highly abundant targets. It is also used for nucleic acid and protein related blotting.This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane, dilute buffer to 1X in water. Warm solution to 50-70°C and perform 2 to 3 changes (15 minutes each) of boiling stripping buffer.
Biochain Institute Inc Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody, 1200 cm2/PK
Attoglow Western Blot Analysis Kit is an ultra-sensitive chemiluminescent Western blot analysis kit developed by BioChain. It detects protein at attogram (10-18 g) or yoctomole (10-24 M) levels. This kit consists of three parts: Millennium Enhancer, protein interaction system and detection system.
Rockland Immunochemicals Revitablot™ Western Blot Stripping Buffer - MB-085-0500
Revitablot™ Western Blot Stripping Buffer is a uniquely formulated, ready-to-use reagent specifically designed for western blotting. Revitablot™ Western Blot Stripping Buffer is faster and more efficient at stripping of primary and secondary antibodies, including HRP and fluorescent labeled. Blots can be stripped multiple times for the repeated use of membranes. This solution can be used at room temperature. Only incubation of 5-20 minutes needed to strip the membrane. Revitablot™ Western Blot Stripping Buffer is gentle and effective on both nitrocellulose and PVDF western blot membranes.
LICORbio Revert™ 700 Total Protein Stain for Western Blot Normalization (100 mL)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. Revert 700 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 700 nm channel of Odyssey Imaging Systems (or competitor systems) for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. The 100 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 250 mL size is sufficient for 50 mini Western blots.
LICORbio Revert™ 520 Total Protein Stain and Wash Solution Kit (1 Kit)
Revert 520 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 520 nm channel for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. This kit contains 200 mL Revert 520 Total Protein Stain and 400 mL Revert 520 Wash Solution. This kit is sufficient for 20 mini Western blots.
LICORbio Revert™ 520 Total Protein Stain Kits for Western Blot Normalization (200 mL kit)
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 200 mL Kit is enough for 20 mini blots and contains 1 x 200 mL Revert 520 Total Protein Stain, 1 x 400 mL Revert 520 Wash Solution, and 1 x 400 mL Revert Destaining Solution
LICORbio Revert™ 520 Total Protein Stain Kits for Western Blot Normalization (500 mL kit)
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 500 mL Kit is enough for 50 mini blots and contains 1 x 500 mL Revert 520 Total Protein Stain, 1 x 1 L Revert 520 Wash Solution, and 1 x 1 L Revert Destaining Solution
Biolegend Western-Ready™ MES SDS-PAGE Running Buffer (10X)
Western-Ready™ MES SDS-PAGE Running Buffer (10X) Apps: WB; Size: 1 L