Electrophoresis Gel Stains
AdipoGen Hoechst 34580 Solution (5 ml)
Chemical. CAS: 911004-45-0. Formula: C27H29N7 · 3HCl. MW: 560.95. The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. The blue fluorescent Hoechst dye is a cell permeable nucleic acid stain that has multiple applications, including sensitive detection of DNA in the presence of RNA in agarose gels, automated DNA determination, sensitive determination of cell number and chromosome sorting. Useful vital stain for the flow cytometric recognition of DNA damage and other viability measurements by monitoring the emission spectral shifts of the dyes. Because this fluorescent stain labels DNA, it can also be used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and an excellent tool for flow cytometry studies.
Revvity Health Sciences Inc IVISense Cat B 750 FAST Fluorescent Probe
IVISense Cat B 750 FAST Fluorescent Probe
Crescent Chemical Co Inc COOMASSIE BRILLIANT BLUE
Coomassie Brilliant Blue G 250 Corresponds to SERVA Blue G (cat. no. 35050). Size - 25G Storage Conditions - +15 C TO +30 C Catalog Number - 17524.01 CAS 6104-58-1
Crescent Chemical Co Inc COOMASSIE BRILLIANT BLUE
Coomassie Brilliant Blue R 250 Corresponds to SERVA Blue R (cat. no. 35051). Size - 25G Storage Conditions - +15 C TO +30 C Catalog Number - 17525.01 CAS 6104-59-2
Sigma Aldrich Fine Chemicals Biosciences Ethidium bromide BioReagent, for molecular biology, powder | 1239-45-8 | MFCD00011724 | 25G
Ethidium bromide BioReagent, for molecular biology, powder | Mol Wt: 394.31 | 1239-45-8 | MFCD00011724 | 25G
Bioworld Loading Buffer (5x), 30% Glycerol, 5 mL
For agarose gels. Excellent electroohoretic dye for most DNA and RNA application. Ultra Pure Grade Contains: 30% Glycerol DNase: None Detected RNase: None Detected Stability (+4°C) >1 year Storage: 2-8°C
Bioworld Rapid Coomassie Stain, 100 mL
Item# 21470008This stain is fast and sensitive in staining proteins in acrylimide gels. Its features include: a) completion within 30-60 minutes, b) unnecessary destaining, c) being highly sensitive, it detects as little as 30 ng of protein, d) yields sharper bands than those stained with conventional Coomassie Blue, and e) gels can be restained with Silver. Solution provided as 2x concentrate.
Bioworld Coomassie Blue R-250 (2X), 500 mL
Stain for location and detection of proteins. Protocol: After SDS-PAGE, transfer the gels in fixing solution (30% methanol and 7% acetic acid) for 30 min. under constant agitation. Replace the fixing solution, submerge the gel in stain and shake the gel gently gor 2-4 hrs. Pour off the stain, rinse the gel briefly with fixing solution and cover the gel with fresh fixing solution for 30 min. Repeat and continue destaining until distinct blue bands against a clear background are obtained. Store the gel in 7% acetic acid solution. Photograph or dry the gel according to your standard protocol.Synonym: Brilliant blue R; Coomassie brilliant blue R-250; Acid Blue 83; CI 42660Appearance: Liquid
Bioworld Coomassie Blue G-250 (2X), 500 mL
A commonly used analytical biochemistry dye and P2X7 antagonistStain for location and detection of proteins. Protocol: After SDS-PAGE, transfer the gels in fixing solution (30% methanol and 7% acetic acid) for 30 min. under constant agitation. Replace the fixing solution, submerge the gel in stain and shake the gel gently gor 2-4 hrs. Pour off the stain, rinse the gel briefly with fixing solution and cover the gel with fresh fixing solution for 30 min. Repeat and continue destaining until distinct blue bands against a clear background are obtained. Store the gel in 7% acetic acid solution. Photograph or dry the gel according to your standard protocol.Synonym: Synonym: Acid blue 90, Coomassie Brilliant Blue GAppearance: LiquidHS Code : 32041200
Bioworld Coomassie Brilliant Blue R-250, 10 g
Protein electrophoresis dye used in SDS-PAGE.More sensitive than G-250.Ultra Pure GradeEM (550nm, water) >33,000Protein Staining: PassesAppearance: Solid
Bioworld Express Coomassie Stain, G-250, 500 mL
Express Coomassie Stain detects as little as 15 ng of protein and gives complete band detection within 50 minutes. Its ideal for densitometry and its able to be restained with silver. This stain can be used more than once. Express Coomassie is a colloidal suspension of Coomassie blue that preferentially binds to protein and eliminates the unwanted destaining step. Available in both R-250 and G-250.Appearance: LiquidHS Code : 32041200Melting Point: 100 °C
AdipoGen Ethidium homodimer Solution
Chemical. CAS 61926-22-5. Formula C46H50Cl4N8. MW 856.75. Synthetic. Staining dye for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. It does not cross intact cell membranes and can be used to test cell viability. Reagent for the fluorimetric detection of nucleic acids. Spectral data lambdaex=528nm, lambdaem=617nm.
Bioworld Ethidium Bromide, 25 g
Biotechnology GradeA fluorescent electrophoretic stain for nucleic acidsAlso used as a nucleic acid intercalating agent and frameshift mutagenAmmonium bromide Concentration: <0.1%Regulated SubstanceFluoresence: λex 480 nm, λem 620 nm in waterHaz Mat fee appliesAppearance: SolidHS Code : 29339990Melting Point: 260-262 °C (dec.)(lit.)RTECS Number: SF7950000
Alkali Scientific SmartGlow™ Safe Green Pre Nucleic Acid Stain, Loading Dye, 1mL
SmartGlow Nucleic Acid Stain is ideal for excitation of nucleic acids with UV or visible blue light transilluminators. Unlike EtBR, SmartGlow it is non-mutagenic and does not require special methods of disposal. SmartGlow PS (Pre Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA, ssDNA and RNA. SmartGlow PS exhibits excitation peaks at 290nm and 490nm, allowing it to be used with UV and blue light. SmartGlow LD (Loading Dye) is supplied at 6X concentration and is added directly to the samples. No dye needs to be added to the gel or running buffer. After electrophoretic separation, view and document your results using a UV or blue light illuminator. SmartGlow LD emits green fluorescence when bound to dsDNA, ssDNA and RNA. Replaces hazardous EtBr Excitation by UV or blue light Non-mutagenic, environmentally safe Two types: PS (Pre-Stain) LD (Loading Dye)