PCR Equipment and Supplies
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Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Rat ACTB (actin, beta) Endogenous Control (VIC™/MGB probe, primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Rat ACTB (actin, beta) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
| Product Type | Genotyping Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 |
| Sample Type | DNA (Genomic) |
| Reaction Speed | Standard |
| Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
| Product Line | TaqMan |
Applied Biosystems™ Rat GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Rat GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Content And Storage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Form | Liquid |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 5-plex |
| Detection Method | Primer-probe |
| Includes | dUTP |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Antibody-based |
| Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Product Line | TaqPath |
Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Type | Universal Master Mix II |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Product Line | TaqMan |
Invitrogen™ Taq DNA Polymerase PCR Buffer (10X)
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl2.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ DreamTaq™ Buffers (10X)
Thermo Scientific 10X DreamTaq Buffer is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase.
| Quantity | 4 x 1.25 mL |
|---|---|
| Concentration | 10X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 to 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Thermo Scientific™ DNA Polymerase I (10 U/μL)
DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.
| Polymerase | DNA Polymerase I |
|---|---|
| Description | DNA Polymerase I |
| Concentration | 10 U/μL |
Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Gene Symbol | 18S |
| Amplicon Size | 187 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | X03205.1 |
| Target | 18S rRNA |
| Format | Tube |
| Label or Dye | FAM |
| Species | Eukaryotic, Rat, Mouse, Human |
| Product Line | FAM, TaqMan |