PCR Equipment and Supplies
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Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Activation Time | 5 min. |
| Final Product Type | Mastermix |
| Form | Liquid |
| Reaction Speed | Fast |
| Label or Dye | ROX |
| Hot Start | Built-in Hot Start |
| Concentration | 2X |
| Starting Material | DNA/RNA |
| Compatibility | TaqMan™ |
| For Use With (Application) | Animal Health |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment
DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | DNA Polymerase I |
|---|---|
| Content And Storage | DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C. |
| Hot Start | No |
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phusion U Multiplex PCR Master Mixes
Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 2.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Multiplex PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Reverse Transcriptase | M-MLV |
| Purification Time | 5 min. |
| Concentration | 20X |
| For Use With (Application) | RT-PCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Cells |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Hot Start | Built-In Hot Start |
| Product Line | Cells-to-CT, SYBR |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Super Mix |
| Reverse Transcriptase | SuperScript III |
| Reaction Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Anchored Oligo(dT)20 Primer
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Reagents Starter Kit
TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sample Type | DNA (Genomic), cDNA |
|---|---|
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Frozen, Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Form | Liquid |
| Isolation Technology | Direct Cell Lysis |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | Yes |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Target | cDNA |
| Format | Kit |
| Product Type | Direct Cell Lysis to RT-qPCR Kit |
| Sample Type | Hepatocytes, HepaRG, HepG2 |
| Reaction Speed | 10 min. |
| Label or Dye | ROX |
| Purification Target | RNA |
| Starting Material | Up to 100,000 Cultured Cells |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript II |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 to 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ Oligo d(T)16 (50 μM)
Invitrogen Oligo(dT)16 Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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