PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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211 – 225 of 8,347 results

211

Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Pricing & Availability

212

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Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

213

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Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

Pricing & Availability

214

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Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Pricing & Availability

215

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	LD (Low DNA)
Reverse Transcriptase	M-MLV
Purification Time	5 min.
Concentration	20X
For Use With (Application)	RT-PCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cells
Reaction Speed	Standard
Label or Dye	SYBR Green
Hot Start	Built-In Hot Start
Product Line	Cells-to-CT, SYBR

216

Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Pricing & Availability
Specifications

Polymerase	DNA Polymerase I
Content And Storage	DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂ , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
Hot Start	No

217

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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	120 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Quantity	1 Ea.
Technique	Reverse Transcription
Concentration	50 U/μL
Starting Material	RNA

218

Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

Pricing & Availability

219

Thermo Scientific™ dITP Solution (100 mM)

Thermo Scientific dITP (2'-deoxyinosine 5'-triphosphate) is a highly stable nucleotide supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

220

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

221

Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Form	Liquid
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	HRM
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Genotyping Target	SNPs (Unknown or Numerous), Epigenetic Targets
Product Type	HRM Master Mix
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Label or Dye	High Resolution Melting (HRM),SYTO 9
Technique	HRM (High Resolution Melting) Analysis, Mutation Scanning
Product Line	MeltDoctor

222

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Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Pricing & Availability
Specifications

Fidelity (vs. Taq)	1X
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Overhang	3'-A
Concentration	5 U/μL
Starting Material	DNA
Exonuclease Activity	5' - 3'

223

Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR

224

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Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Starting Material	DNA

225

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing & Availability

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Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase