PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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241 – 255 of 8,322 results

241

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Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan
Detection Method	Primer-probe

242

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Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Pricing & Availability

243

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Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Hot-start PCR

244

Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

Pricing & Availability

245

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Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Format	Stand-alone Enzyme
Reverse Transcriptase	M-MLV
Size (Final Product)	Up to 7 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Concentration	200 U/μL
Starting Material	RNA

246

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Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	1-step RT-PCR Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 13 kb
Reaction Format	Master Mix
Reagent Type	Reverse Transcription
Hot Start	Built-in Hot Start
Optimal Reaction Temperature	50°C to 65°C
Technique	1-step RT-PCR
Starting Material	RNA
PCR Method	1-step RT-PCR
Multiplex Capability	Yes
Detection Method	Gel Electrophoresis

247

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Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

248

Invitrogen™ SYBR™ Green Cells-to-C_T™ Control Kit

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-C_T™ Kits with sufficient reagents are provided for 100 reactions.

Pricing & Availability

249

Invitrogen™ T4 DNA Polymerase

DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Polymerase	T4 DNA Polymerase
Content And Storage	T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.
Description	DNA Polymerase
Hot Start	No
Exonuclease Activity	3' - 5'

250

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Invitrogen™ Platinum™ II Green PCR Buffer (5X)

Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR

Pricing & Availability

251

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	RevertAid H Minus
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

252

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Cells-to-C_T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-C_T™ Kit (20X RT enzyme mix and 20X RT buffer).

Pricing & Availability

253

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Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit

The TaqMan™ Gene Expression Cells-to-C_T™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Polymerase	DNA Polymerase
Format	Tube
Product Type	Gene Expression Cells-to-C Kit
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cell
Reaction Speed	Standard
Product Line	Ambion, Cells-to-CT, TaqMan

254

Applied Biosystems™ VetMAX™-Plus One-Step RT-PCR Kit

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing & Availability

255

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)₁₈ Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase	M-MLV
Size (Final Product)	7 kb or less
Final Product Type	cDNA
Sample Type	Mammalian Cells
Purification Time	2 hr.
Optimal Reaction Temperature	42°C
For Use With (Application)	Real Time PCR (qPCR),RT-PCR
Product Line	Ambion, Cells-to-cDNA

PCR Equipment and Supplies

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Applied Biosystems™ TaqMan™ Universal PCR Master Mix GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SuperScript™ IV Reverse Transcriptase GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT Bulk RT Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Invitrogen™ SuperScript™ IV Reverse Transcriptase

Invitrogen™ SYBR™ Green Cells-to-C_T™ Control Kit

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit