PCR Equipment and Supplies

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Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Invitrogen™ Oligo (dT) Primer (50 μM)

These are the same Invitrogen primers that are currently included in the SuperScript IV Kit (Cat. No. 18091050).

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Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Frozen, Dry Ice
Target	cDNA
Content And Storage	Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format	Kit
Sample Type	Mammalian Cells
Isolation Technology	Direct cell lysis
Kit Type	Direct Cell Lysis RT-qPCR
Purification Target	RNA
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
High-throughput Compatibility	Yes
PCR Method	2-step RT-qPCR
Detection Method	TaqMan

Applied Biosystems™ Detection Enhancer, for real-time PCR

Formulated to improve template amplification in real-time PCR (qPCR) workflows

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Product Type	Detection Enhancer
For Use With (Equipment)	7500 System
Sample Type	Viral Samples
Reaction Speed	Standard
For Use With (Application)	Real Time PCR (qPCR)
PCR Method	1-step RT-qPCR
Product Line	Applied Biosystems
Detection Method	Primer-probe

Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls

VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.

Pricing & Availability

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Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	ArrayScript™ UP
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	RNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Run Time	Standard
Product Line	RNA-to-C_T, SYBR

TaqMan™ PGx Express Panel, 384-well plate

This TaqMan PGx (Pharmacogenomics) Express Panel is offered in pre-spotted 384-well plates, an efficient, easy-to-use format for pharmacogenomics applications.

Pricing & Availability

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Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Master Mix
Final Product Type	First-Strand cDNA
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Technique	Template Switching Reverse Transcription
Concentration	4X
Starting Material	Cells or RNA

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Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	RevertAid H Minus
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)₁₈ Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase	M-MLV
Size (Final Product)	7 kb or less
Final Product Type	cDNA
Sample Type	Mammalian Cells
Purification Time	2 hr.
Optimal Reaction Temperature	42°C
For Use With (Application)	Real Time PCR (qPCR),RT-PCR
Product Line	Ambion, Cells-to-cDNA

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 6-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	None
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath

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Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch

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Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

Pricing & Availability
Specifications

Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR

Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Separation Range	100 bp to 12 kb
Content And Storage	Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume	125 μL
Isolation Technology	Silica Spin Column
Elution Volume Max	20 μL
For Use With (Application)	Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Target	DNA from Enzymatic Reactions (e.g. PCR)
Format	Spin Column
Product Type	PCR Micro Kit
Run Time	10 min.
Elution Volume Min	5 μL
Product Line	PureLink

Thermo Scientific™ dGTP Solution (100 mM)

Thermo Scientific dGTP (2'-deoxyguanosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

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Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ dGTP Solution (100 mM) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

Thermo Scientific™ dGTP Solution (100 mM)