PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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136

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Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Pricing & Availability
Specifications

Polymerase	Platinum Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Starting Material	DNA
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'

137

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Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type	Genotyping Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type	DNA (Genomic)
Reaction Speed	Standard
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line	TaqMan

138

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	High
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	Direct PCR
Polymerase	Phusion Blood II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

139

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Thermo Scientific™ DNA Polymerase I (10 U/μL)

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Pricing & Availability
Specifications

Polymerase	DNA Polymerase I
Description	DNA Polymerase I
Concentration	10 U/μL

140

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Applied Biosystems™ TaqMan™ β-Actin Detection Reagents

TaqMan™ β-Actin Detection Reagents

Pricing & Availability

141

Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

Pricing & Availability

142

Invitrogen™ Platinum™ Taq DNA Polymerase, High Fidelity

Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	6X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
For Use With (Application)	Hot-start PCR,High-fidelity PCR
Detection Method	Primer-probe

143

Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
PCR Method	qPCR,Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	Taq DNA Polymerase
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

144

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	2X
For Use With (Application)	Cloning,Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold DNA Polymerase
Size (Final Product)	1.5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Fast PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

145

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch

146

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	1-step RT-PCR Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 13 kb
Reaction Format	Master Mix
Reagent Type	Reverse Transcription
Hot Start	Built-in Hot Start
Optimal Reaction Temperature	50°C to 65°C
Technique	1-step RT-PCR
Starting Material	RNA
PCR Method	1-step RT-PCR
Multiplex Capability	Yes
Detection Method	Gel Electrophoresis

147

Invitrogen™ Taq DNA Polymerase PCR Buffer (10X)

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl₂.

Pricing & Availability

148

Invitrogen™ SuperScript™ III First-Strand Synthesis System

The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)⁺ or total RNA.

Pricing & Availability

149

Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Format	Stand-alone Enzyme
Reverse Transcriptase	M-MLV
Size (Final Product)	Up to 7 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Concentration	200 U/μL
Starting Material	RNA

150

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq DNA Polymerase
Format	One-Step RT-PCR System
Size (Final Product)	Up to 4.5 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

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PCR Supplies

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Thermo Scientific™ DNA Polymerase I (10 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ DNA Polymerase I (10 U/μL)