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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (2,083)
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DNA Polymerases (1,059)
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Keyword Search:
136 – 150 of 4,122 results
136

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
137

Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Format Tube
Passive Reference Dye ROX (Pre-mixed)
Form Liquid
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Hot Start Built-In Hot Start
For Use With (Application) Animal Health,Pathogen Detection
PCR Method qPCR
Product Line Ambion, Path-ID
Detection Method Primer-probe
138

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

EDGE ENCOMPASS_PREFERRED
139

Applied Biosystems™ SYBR™ Select Master Mix for CFX

A cost-effective solution for real-time PCR applications.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Product Line SYBR
140

Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
141
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

EDGE ENCOMPASS_PREFERRED
Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
142
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG
GSA_VA

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
143
Promotions Available

Applied Biosystems™ Power SYBR™ Green PCR Master Mix
GSA_VA

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Purity or Quality Grade LD (Low DNA)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Label or Dye SYBR Green
Product Line SYBR
144

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Reverse Transcriptase M-MLV
Form Liquid
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Thermostability Not Applicable
Polymerase AmpliTaq Fast DNA Polymerase
Format Tube
Product Type 1-Step Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqMan
145
Promotions Available

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
GSA_VA

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line TaqMan
146

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE ENCOMPASS_PREFERRED
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
147

Invitrogen™ SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type PCR Amplified cDNA
Quantity 1 Ea.
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum SuperFi
Format Kit
Size (Final Product) Up to 14 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 45°C to 60°C
Technique 1-step RT-PCR
Starting Material RNA
148

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
GSA_VA

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq High Fidelity
Format One-Step RT-PCR System
Size (Final Product) Up to 10 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
149

Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

EDGE ENCOMPASS_PREFERRED
150

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
GREENER_CHOICE

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

EDGE ENCOMPASS_PREFERRED
Fidelity (vs. Taq) 1X
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Overhang 3'-A
Concentration 5 U/μL
Starting Material DNA
Exonuclease Activity 5' - 3'