PCR Supplies
Bio Rad Laboratories SSOADVANCED UNIVERSAL PROBES S
SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
Sigma Aldrich Fine Chemicals Biosciences Mix-n-StainTM CfTM 488A An
This labeling kit is for labeling 20-50 mug antibody with the green fluorescent dye Cf488A. Mix-n-Stain Cf dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior Cf dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Cf488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection settings Cf488A is similar to Alexa fluor(R) 488 in brightness and photostability. In microscopy or flow cytometry applications using a 520/20 filter Cf488A may appear brighter than Alexa fluor(R) 488 due to its shorter emission wavelength which makes the dye optically more compatible. The shorter wavelength of Cf488A also minimizes the dyes spill-over into longer wavelength channels making the dye advantageous in multi-color detection. Like Alexa fluor(R) 488 Cf488A is highly water soluble but is not
Roche Diagnostics HotStart ReadyMix with dye, 500 x 25 uL reactions, 6.25 mL
HotStart ReadyMix with dye, 500 x 25 uL reactions, 6.25 mL. *This item is non returnable.
Roche Diagnostics KAPA HiFi HotStart Uracil+ ReadyMix Kit (50 rxn), 50 x 50 uL reactions
KAPA HiFi HotStart Uracil+ ReadyMix (2X) contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM). *This item is non returnable.
Roche Diagnostics KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions
KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions. *This item is non returnable.
Roche Diagnostics KAPA SYBR FAST qPCR Kit | 100x20uL Reactions (Universal)
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2at a final concentration of 2.5 mM. *This item is non returnable.
New England Biolabs, Inc. T4 DNA Polymerase – 150 units
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5' to 3' direction and requires the presence of template and primer. This enzyme has a 3' to 5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5' to 3' exonuclease function.
New England Biolabs, Inc. Deoxynucleotide (dNTP) Solution Mix – 8 µmol of each
Deoxynucleotide (dNTP) Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP. A small pack contains 4 vials of 0.2 mls and a large pack contains 5 vials of 0.8 mls. Supplied in: Ultrapure water as a sodium salt at pH 7.5.
- Compatible with all NEB DNA polymerases in a variety of applications
- >= 99% pure as determined by HPLC analysis
- Exceptional stability
New England Biolabs, Inc. Taq DNA Polymerase with ThermoPol Buffer – 2000 units
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 53 polymerase activity and a 5 flap endonuclease activity. It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
Roche Diagnostics LightCycler 480 SYBR Green I Master
LightCycler 480 SYBR Green I Master - 5 x 1 ml (2x conc.) (approx. 500 reactions of 20uL final reaction volume)
Takara Bio TAQ POLYM HOT-START 1000U
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 1000U (4 x 250U); reduces background, increased specificity, permits room-temperature reaction assembly
New England Biolabs, Inc. Deep Vent™ DNA Polymerase – 200 units
Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3 5 proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100C.
New England Biolabs, Inc. phi29 DNA Polymerase – 250 units
phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29. This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3' to 5' proofreading exonuclease activity.