PCR Supplies
Biotium Fast Probe Master Mix, trial size (100 rxn)
Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq. Cheetah™ Taq is a chemically modified hotstart Taq which can be fully activated in 2 minutes, thus permitting fast PCR. This product contains no ROX reference dye and has sufficient reagent for 100 reactions.
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix with UDG – 100 reactions
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
New England Biolabs, Inc. Q5 High-Fidelity PCR Kit – 200 reactions
Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions or 200 reactions. For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
Apexbio Technology LLC 2XFAST GR QPCR MASTER MIX 50ML
2XFAST GR QPCR MASTER MIX 50ML APEXBIO TECHNOLOGIES
Sigma Aldrich Fine Chemicals Biosciences fastStart TaqManR Probe Ma
Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore there is no elongation during the period when primers can nonspecifically bind. The fastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
GenDEPOT amfiSure ONE PCR Master Mix(2X), P7000, 5 x 1 mL
amfiSure ONE PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure PCR Master Mix provides superior yields in both routine and challenging PCR application such as direct PCR using crude extracted DNA, GC rich region PCR, colony PCR, multiplex PCR and etc.
Sigma Aldrich Fine Chemicals Biosciences KOD OneTM PCR Master Mix -
KOD One(TM) PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator enabling fast PCR with an extension time of 5 sec/ kb for template DNA 10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5-3 polymerase activity and 3-5 exonuclease activity. Upon heat-activation the 3-5 proof-reading ability of the enzyme generates blunt-end PCR products.
New England Biolabs, Inc. WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)- 500 rxn
The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with multiple detection methods including turbidity detection real-time fluorescence detection and end-point visualization.
Apexbio Technology LLC 2X PROBE QPCR MASTER MIX 5ML
2X PROBE QPCR MASTER MIX 5ML APEXBIO TECHNOLOGIES
Medchemexpress LLC HY-K0521 1 mL (100 rxns) Medchemexpress, SYBR Green qPCR Master Mix (High ROX) CAS: Purity:>98%
Medchemexpress, HY-K0521 1 mL (100 rxns) SYBR Green qPCR Master Mix (High ROX) CAS: Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Alkali Scientific Radiant™ Green qPCR 2x qPCR Blue Mix, 10000 Reactions, 100 x 1.0mL, Lo ROX
Radiant™ Green qPCR 2x qPCR Blue Mix, 10000 Reactions, 100 x 1.0mL, Lo ROX, provides a highly sensitive solution for PCR amplification. This mix is designed for quantitative PCR assays, offering excellent efficiency and performance. The Lo ROX compatibility ensures minimal background noise, making it ideal for precise and accurate measurements. The green color improves the visual cue for reaction progress, ensuring high-quality results with every cycle.
Alkali Scientific Radiant™ Probe qPCR Mix, Separate ROX, 500 Reactions, 5 x 1.0mL
Radiant™ Probe qPCR Mix, Separate ROX, 500 Reactions, 5 x 1.0mL, is a high-efficiency mix designed for real-time PCR analysis. The separate ROX feature ensures reliable signal normalization, while the 2x formulation provides optimal amplification efficiency. This product is ideal for high-throughput gene expression analysis, offering both sensitivity and accuracy. Whether you're studying gene expression or detection, this mix is an excellent choice for a range of PCR applications.
INTACT GENOMICS INC ig SYBR Green qPCR 2X Master Mix, 5000 Reactions, 10x5ml
The SYBR Green qPCR 2X master mix is a ready-to-use cocktail for the amplification and detection of DNA in qPCR, containing all necessary components except primers and template.
- Enhanced efficiency, specificity, and sensitivity
- Compatibility with all major real-time PCR instruments
- Superior gene expression results under various cycling conditions
- Ready-to-use convenience
- More affordable price
- Includes an integrated chemically modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs, and stabilizers
- Suitable for high-throughput real-time PCR screening and validation
- High-quality hot start Taq DNA polymerase ensures higher fidelity and improved amplification