Lyo-Ready 1-Step RT-qPCR Buffer is a glycerol-free qPCR mix containing RNase Inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients. In order to produce room temperature lyophilized RT-qPCR reagents, Reverse Transcriptase, Taq polymerase, assay specific primers and probes are added to Lyo-Ready 1-Step RT-qPCR Buffer for subsequent lyophilization.