High Fidelity PCR Reagents and Kits
LGC GENOMICS LLC (Lucigen) FAILSAFE PCR PREMIX C 2.5MLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.
Trusted: The same great performance as the FailSafe PCR System, but available as individual componentsFlexible: Purchase any combination of FailSafe 2X PreMixesThis listing is only for reordering of individual FailSafe PCR 2X PreMixes.Before purchasing these separate components, we strongly recommend first optimizing your PCR conditions using the FailSafe PCR PreMix Selection Kit (Item ID. FS99060) in order to know which components to purchase.
New England Biolabs, Inc. Phusion High-Fidelity PCR Master Mix with GC Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with GC Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
LGC GENOMICS LLC (Lucigen) FailSafe PCR 2X PreMix L, 2.5 mLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.
Trusted: The same great performance as the FailSafe PCR System, but available as individual componentsFlexible: Purchase any combination of FailSafe 2X PreMixesThis listing is only for reordering of individual FailSafe PCR 2X PreMixes.Before purchasing these separate components, we strongly recommend first optimizing your PCR conditions using the FailSafe PCR PreMix Selection Kit (Item ID. FS99060) in order to know which components to purchase.
New England Biolabs, Inc. Phusion™ High-Fidelity PCR Master Mix with HF Buffer – 100 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
Sigma Aldrich Fine Chemicals Biosciences ExpandTM High fidelity PCR
The 5 - 3 polymerase activity of Taq DNA Polymerase and the 3 - 5 exonuclease of the proofreading polymerase combine for improved performance and results 3 - 5 exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
LAMDA BIOTECH INC High Fidelity PCR 2x Master Mix
The Ready-to-use HFL PCR Master Mix is for high fidelity and long fragment PCR, which contains modified high fidelity thermal stable DNA polymerases in a pre-optimized PCR buffer. Applying the extra high fidelity thermal stable DNA polymerases in the master mix ensures not only the highest fidelity for the PCR product, but also improves the amplification length of the resulting DNA fragments and the robust amplification efficiency. Amplified DNA contains blunt-ended DNA fragments. The Master Mix is useful when cloning large DNA fragments at high fidelity; such as, promoter and other important DNA regions. The HFL PCR Master Mix is in the 2X format. For most of the PCR experiments with this master mix, only template, primers and H2O will be needed.
Apexbio Technology LLC hyPerFUsion™ high-fidelity PCR Kit, 100U
hyPerFUsion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, hyPerFUsion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, hyPerFUsion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. hyPerFUsion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Other sizes are available. Please inqury us for quote.
Roche Diagnostics KAPA HiFi HotStart PCR Kit (100 U), 100 x 50 uL reactions
KAPA HiFi HotStart PCR Kit (100 U), 100 x 50 uL reactions. *This item is non returnable.
New England Biolabs, Inc. Phusion™ High-Fidelity PCR Master Mix with HF Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Q5 High-Fidelity PCR Kit – 50 reactions
Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions or 200 reactions. For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
Takara Bio ADVANTAGE 2 PCR ENZYME SYSTEM
Advantage 2 PCR Enzyme Kit, 30 rxns, An optimized PCR enzyme mix for use in applications that involve longer templates or require increased fidelity and higher PCR performance
New England Biolabs, Inc. Q5 Blood Direct 2X Master Mix - 100 rxns
Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood anti-coagulants and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA K2 EDTA Na citrate and Na heparin as well samples stored on common preservative filter papers. The master mix features Q5 Hot Start High-Fidelity DNA Polymerase a thermostable DNA polymerase with 3 prime to 5 prime exonuclease activity fused to a processivity-enhancing Sso7d domain for robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.
Roche Diagnostics KAPA HiFi HotStart Library Amplification Kit (250 rxn), 250 x 50 uL reactions
KAPA HiFi HotStart Library Amplification Kit (250 rxn), 250 x 50 uL reactions. *This item is non returnable.
Apexbio Technology LLC hyPerFUsion™ high-fidelity PCR Kit, 500U
hyPerFUsion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, hyPerFUsion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, hyPerFUsion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. hyPerFUsion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Other sizes are available. Please inqury us for quote.