Hot Start PCR Reagents and Kits
Takara Bio TaKaRa Taq™ DNA Polymerase Hot Start Version 250 units
An antibody-mediated hot-start version of TaKaRa Taq DNA Polymerase, which is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tubes of buffer (Mg2+ plus) and dNTPs. 250 units
Boca Scientific Inc SD Polymerase Hotstart, 10 U/ul, 1000 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
Boca Scientific Inc SD Polymerase Hotstart, 10 U/ul, 200 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
ABclonal Technology TruePol 2X PCR Mix for Microbiome
Truepol 2X PCR Mix for Microbiome is developed for microbial diversity detection, having high amplification efficiency for various types of samples. Certain specific components mixted in this reagent enhance inhibitor tolerance and hence to improve the sensitivity. Even there is large amount of host DNA as interference in samples, Truepol 2X PCR Mix for Microbiome ensures good amplification efficiency, significantly improving the success of microbial detection rate. Truepol 2X PCR Mix for Microbiome can be used for dealing with general and complex samples, such as human tissue, soil, feces, urine, air filter membrane, sand, etc.
Apexbio Technology LLC HyperScript RT SuperMix for qPCR (with gDNA wiper) 100 rxns
HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration
Thomas Scientific Hot-Start TaqT DNA Polymerase, 5u/æl, Pack: 1000u
Inactive at room temperature Increased specificity This is the Hot-Start version of Choice-Taq™ DNA Polymerase that requires a pre-activation step at 95°C for 5 minutes in order to achieve a fully functional polymerase enzyme. The inactivity of the enzyme at room temperature increases the specificity of the enzyme for the desired template.
ABclonal Technology Gloria U 2X HS Master Mix
Gloria U Hot-Start DNA Polymerase is a DNA polymerase obtained through molecular modification, which can effectively amplify templates containing uracil. The uracil-binding ability of Gloria U polymerase is inactivated, allowing it to efficiently amplify DNA that has been treated with bisulfite. Moreover, this enzyme exhibits the same high yield and low GC bias as Gloria Nova Hot-Start DNA Polymerase. Gloria U Hot-Start High-Fidelity DNA Polymerase possesses 5′-3′ polymerase and 3′to5′ exonuclease (proofreading) activities, but lacks 5′-3′ exonuclease activity. The Gloria U 2X HS Master Mix contains Gloria U DNA Polymerase, dNTPs, 5X Gloria U Reaction Buffer, and other components. Only template and primers need to be added.
Cytek Biosciences Inc CLASSIC++ HOTSTART TAQ MIX200R
Classic++ Hot Start Taq DNA Polymerase Master Mix Apps: PCR Size: 200 rxns
ABclonal Technology TruePol 2X PCR Mix for Microbiome
Truepol 2X PCR Mix for Microbiome is developed for microbial diversity detection, having high amplification efficiency for various types of samples. Certain specific components mixted in this reagent enhance inhibitor tolerance and hence to improve the sensitivity. Even there is large amount of host DNA as interference in samples, Truepol 2X PCR Mix for Microbiome ensures good amplification efficiency, significantly improving the success of microbial detection rate. Truepol 2X PCR Mix for Microbiome can be used for dealing with general and complex samples, such as human tissue, soil, feces, urine, air filter membrane, sand, etc.
ABclonal Technology Gloria Nova HS 2X PCR Mix for NGS
Gloria Nova HS DNA Polymerase is a high-fidelity engineered enzyme rivaling Pyrococcus furiosus DNA polymerase. Featuring a synthesis-enhancing domain, it delivers fast extension speeds and ultra-high fidelity (10–15× Taq, 6× P. furiosus), aided by strong 3’to5’ proofreading. Its antibody-mediated hot-start prevents nonspecific amplification. The kit includes 5X HF Buffer (general high-fidelity PCR) and 2.5X GC Buffer (GC-rich/problematic templates), ensuring robust performance across diverse applications.