Hot Start PCR Reagents and Kits
Agilent Technologies PFUULTRA II HOTSTART PCR MASTE
PfuUltra II Hotstart PCR Master Mix is a 2X formulation of PfuUltra II Fusion HS DNA polymerase, an optimized PCR reaction buffer, magnesium, and dNTPs.
101 BIO 2x GoldStar Best PCR Master Mix (with dye), (1 mL, 100 rxn, 20 ul/rxn)
2x GoldStar Best PCR Master Mix (with dye) is a convenient premixed 2x concentrated solution for PCR which include GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier.The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity. It also possesses 3´→5´ exonuclease (proofreading) activity. The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase.The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation. Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity.
New England Biolabs, Inc. FauI – 200 units
A restriction endonuclease that recognizes the sequence CCCGCNNNN^NN_.
LAMDA BIOTECH INC HotStart PCR 2x Master Mix in Red
Ready-to-use 2X hot start PCR master mix in green color, containing a HotStart Taq DNA polymerase and a PCR enhancing DNA polymerase. Users only need to add template, primers and H2O for the reaction. The HotStart PCR Master Mix contains inert, non-toxic dyes to make the color in green, which makes it easy to visualize the PCR mixing step, and also allows direct loading of PCR products for electrophoresis. The HotStart Taq DNA Polymerase is a chemically modified Taq DNA Polymerase, whose enzyme activities can only be activated after 3-5 minutes of incubation at 95° C. The HotStart Taq Polymerase uses amplification conditions for regular Taq DNA Polymerase, except no polymerase activity will be present before the onset of thermal cycling. This prevents nonspecific DNA amplification and primer dimer formation. The amplified products, up to 7Kb in length, contain PCR products with blunt end and 3’-overhanging A end.
Sigma Aldrich Fine Chemicals Biosciences PCR Buffer Without MgCl2 1
The PCR buffer without MgCl2 in combination with the MgCl2 stock SOLUTIONon is used for the individual adjustment of the Mg2 concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200muM each. In many cases however it is required to titrate the optimal Mg2 concentration to increase the specificity and yield of the PCR reaction.
New England Biolabs, Inc. LongAmp Hot Start Taq 2X Master Mix – 500 reactions (50 µl vol)
LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep Vent DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp Hot Start Taq 2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3' tp 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq. LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.
Clontech Labs 3P EMERALDAMP MAX 800 RXN
PCR Master Mix, EmeraldAmp Max; 800 x 50uL rxns; Includes optimized buffer, high-yield PCR enzyme, dNTP mixture, gel loading dye (green) and density reagent in 2X premix; High yield: 10X more end product
New England Biolabs, Inc. LongAmp Hot Start Taq DNA Polymerase – 2500 units
LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep Vent DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase. LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient Quick-Load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Clontech Labs 3P TAQ POLYM HOT-START 1000U
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 1000U (4 x 250U); reduces background, increased specificity, permits room-temperature reaction assembly
Clontech Labs 3P EX TAQ POLYM HOT-START 250U
PCR Reagents; Takara; Hot-Start Ex Taq DNA Polymerase; 250U; reduces background, increased specificity, permits room-temperature reaction assembly
Thomas Scientific Hot-StartT Taq Blue MastermixT, 1.5mM MgCI2, 400 reactions
Hot-Start Taq Mastermix™ is the heat activated version of Denville's 2X, ready to go reaction mixture. It is inactive at room temperature and only requires addition of template, primers, and water. A 5 minute pre-heat step at 95ÝC is required to successfully carry out primer extensions. The Blue version contains a non-toxic, non-hazardous inert blue dye, which allows users to ensure adequate mixing and acts as a loading buffer following completion of the PCR reaction. Prices are based on 50ul reactions.
Cytek Biosciences Inc CLASSIC++ HOTSTART TAQ MIX200R
Classic++ Hot Start Taq DNA Polymerase Master Mix Apps: PCR Size: 200 rxns