Long Range PCR Reagents and Kits
Meridian Life Science Tissue Extract-PCR Buffers, 1ML
Tissue Extract-PCR Buffer offers a convenient, fast and efficient method for the extraction of DNA from a variety of mammalian tissues, particularly from rodent tail or ear samples. The DNA extractions are performed in a single tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination, and can be used directly in a PCR reaction. Use with high quality Taq HS DNA Polymerase (MDX008) or inhibitor tolerant qPCR mixes for improving yield of even the most challenging targets
INTACT GENOMICS INC Sau DNA Polymerase Large Fragment 200 Units (5 units/µl)
Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.Applications Strand displacement DNA synthesis (1) Random primer labeling Second strand cDNA synthesis dA-tailing
AdipoGen BioStab PCR Optimizer
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5´-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. BioStab PCR Optimizer solves this problem and outperforms betaine. The mode of action of BioStab PCR optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets e.g. sequences with >70% GC pairs. BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution.
AdipoGen BioStab PCR Optimizer
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5´-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. BioStab PCR Optimizer solves this problem and outperforms betaine. The mode of action of BioStab PCR optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets e.g. sequences with >70% GC pairs. BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution.
AdipoGen MgATP Solution
Chemical. Synthetic. Pre-coupled Mg-ATP is an ideal energy source for semi-purified conjugation/degradation reactions. Supplied as a pH adjusted solution and will not alter reaction pH. Eliminates the reaction needed to produce usable ATP when MgCl2 and ATP are added separately. The amount provided is sufficient for 10 to 50 reactions depending on conditions.