Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
New England Biolabs, Inc. Phusion Hot Start Flex 2X Master Mix – 100 reactions (50 µl vol)
Phusion High-Fidelity DNA Polymerase offers high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. It is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup with the addition of an aptamer-based inhibitor. The formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification.
New England Biolabs, Inc. Luna® Universal Probe One-Step RT-qPCR Kit – 500 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Sigma Aldrich Fine Chemicals Biosciences LookOut Mycoplasma qPCR Detection Kit, 1 kit
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
Sigma Aldrich Fine Chemicals Biosciences LookOut(R) Mycoplasma PCR Detection Kit Optimized for use with JumpStart(TM) Taq DNA Polymerase, D9307. | 1KT
LookOut(R) Mycoplasma PCR Detection Kit Optimized for use with JumpStart(TM) Taq DNA Polymerase, D9307. | 1KT
Takara Bio AAVPRO TITRATION KIT (RT PCR)
6233 \ AAVpro Titration Kit (for Real Time PCR) Ver.2 \ 100 Rxns
TRIBIOSCIENCE INC 4-in-1 Aspergillus qPCR Kit, 100 Reactions
The 4-in-1 Aspergillus qPCR Kit is designed for identifying 4 Aspergillus species of A. flavus, A. fumigatus, A. niger, and A. terreus in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels.
MTC Bio qPCR Sealing Film, Optically Clear, Standard Adhesive, Sterile, 10 bags of 10 seals, 100/pk
PureAmp™ Sealing Films are compatible with all standard PCR plates.Film and foil options include standard adhesive pierceable aluminum, standard adhesive transparent, optically clear standard adhesive, optically clear non-tacky pressure activated adhesive, and low-adhesive EZ-peel, all of which are guaranteed to keep evaporation rates below 5%.• Multiple types for PCR, qPCR, and ELISA• All types are available sterile• Evaporation rates < 5%• 10 inner bags of 10, 100 per pack• Certified RNase, DNase, DNA, & PCR inhibitor free
Takara Bio PREMIX EX TAQ PROBE QPCR 200
qPCR, Probe detection, Premix Ex Taq (Probe qPCR), TaqMan detection
TRIBIOSCIENCE INC Mycoplasma Detection qPCR Kit, 100 Reactions
Mycoplasma Species qPCR Kit has been designed to specifically identify the mycoplasma genus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The kit provides a validated DNA preparation and qPCR procedures for mycoplasma species detection.
Agilent Technologies PFU ULTRA 2-HS2X MASTER
PFU Ultra II Hotstart PCR Master Mix is a 2X formulation of PfuUltra II Fusion HS DNA polymerase, an optimized PCR reaction buffer, magnesium, and dNTPs.
Alkali Scientific Fast Optical qPCR Plates, WHITE, Low profile, Half-Skirted, 96 x 0.1ml Well, ABi Style A1notch, 5 bags of 10 plates, 50/Pk
Fast Optical qPCR Plates, WHITE, Low profile, HalfSkirted, 96 x 0.1mL Well, ABi Style A1notch, 5 bags of 10 plates, 50/pk
Alkali Scientific ProCycle Fast Optical qPCR Plates, Half-Skirted, 96 x 0.1ml Well, H12 notch, 10/Pk
ProCycle Fast Optical qPCR Plates, HalfSkirted, 96 x 0.1mL Well, H12 notch, 10/pk