Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Takara Bio SapphireAmp® Fast PCR Master Mix, 800 Rxns
A hot-start 2X PCR master mix with dye. SapphireAmp Fast PCR mix is well-suited for E.coli-based colony PCR, and colony checks can be completed in about 1 hour. Reactions performed with this mix can be loaded directly onto a gel for electrophoresis. Restriction digestion of PCR products is possible in SapphireAmp reaction buffer. 800 Rxns
Apexbio Technology LLC 2HyPerFUsion High-Fidelity Master Mix, 5ml
Our product 2HyPerFUsion High-Fidelity Master Mix offers superior performance for major PCR applications with high fidelity, powerful amplification ability and faster amplification speed. HyPerFUsion high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes hyPerFUsion high-fidelity DNA polymerase a superior choice for cloning or other subsequent applications. HyPerFUsion high-fidelity DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu). Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC Direct Mouse Genotyping Kit Plus, 200 rxns
The Direct Mouse Genotyping Kit plus provides convenience in faster preparation and superior PCR amplification. Lysis buffer and balance buffer could digest mice tissues rapidly to release unbroken genomic DNA, and it will be used as PCR template directly without extraction from the mixed solution. By using our kit, you can substantially shorten your time for digestion. Furthermore, the 2PCR Master Mix plus (With Dye) will guarantee accurate amplification consequence. Other sizes are also available. Please inqury us for quote.
Cytek Biosciences Inc FastPROBE™ qPCR Hi-ROX Master Mix
FastPROBE™ qPCR Hi-ROX Master Mix ..... Cat. No. 31-5112-0500R ..... Size 500 rxns
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 1000 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
Alkali Scientific Radiant™ Probe qPCR Kit, 5,000 Reactions, (50 x 1mL), HiROX
Radiant™ Probe qPCR Kit Novel hot-start chemistry for improved specificity and sensitivity. Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods
Zymo Research Corporation ZymoTaq qPCR Premix (200 Rxns)
It features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treatedDNA. It also includes an intense dsDNA-specific fluorescentdye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (Cat. No. E2005, E2006, E2007) for low copy detection of DNA from human, bacteria, or fungi.
Alkali Scientific Radiant™ Probe Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Probe Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Alkali Scientific Radiant™ Probe 1Step LoRox qPCR Virus Kit, 4X
cDNA Synthesis and qPCR in a single tubeHighly concentrated 4X mix allows for increased sample and viral input. This optimizes sample sensitivity with small reaction volumes.Engineered for use with a wide range of probe technologies including TaqMan, Scorpions and molecular beacon probesMix includes the thermostable Radiant™ Reverse Transcriptase which is blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNasePlease see product attachment(s)
Enzo Life Sciences EPIXTRACT DNA Isolation Kit for Plasma/Serum (100Reactions)
EPIXTRACT DNA Isolation Kit for Plasma/Serum provides a simple method to isolate DNA from plasma, serum, and body fluids. Assay Time: <15 min.