Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Meridian Life Science qPCR Extraction Control Red, 10ML
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s qPCR Extraction Control Red uses E. coli cells of a known concentration that contain quantitative PCR positive control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target DNA). The cells are added to the sample and lysed and extracted with the sample DNA for downstream amplification by qPCR. The detection of qPCR Extraction Control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
ACROBiosystems E1A&SV40LTA resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Meridian Life Science Inhibitor-Tolerant qPCR Mix 5x, 40ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 5x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that requiremore room for sample, minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Zymo Research Corporation Quick-cfRNA Serum & Plasma Kit
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
Meridian Life Science RT-qPCR Extraction Control Red, 1ML
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s RT-qPCR Extraction Control Red uses artificial micelles that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). The cells are added to the RT-qPCR Extraction Control is spiked in the with lysis buffer into the sample prior to RNA extraction for downstream amplification by RT-qPCR. The detection of RT-qPCR Extraction Control acts as a cDNA synthesis and PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
Sigma Aldrich Fine Chemicals Biosciences Insulin-transferrin-sodium selenite media supplement gamma-irradiated, lyophilized powder, BioXtra, suitable for cell culture | 1VL
Insulin-transferrin-sodium selenite media supplement gamma-irradiated, lyophilized powder, BioXtra, suitable for cell culture | 1VL
Enzo Life Sciences AMPIGENE qPCR 1-Step Green Kit Hi-ROX (1000Reactions)
AMPIGENE qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Lo-ROX, see ENZ-NUC108. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below. Applications: PCR.
Bioland Scientific LLC 2x Taq PCR Premix (5x1 ml, 400 RXN, Green)
An optimized ready-to-use solution containing Taq DNA polymerase, standard Taq reaction buffer, dNTPs, tracking dyes and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Apexbio Technology LLC Direct Mouse Genotyping Kit Plus, 200 rxns
The Direct Mouse Genotyping Kit plus provides convenience in faster preparation and superior PCR amplification. Lysis buffer and balance buffer could digest mice tissues rapidly to release unbroken genomic DNA, and it will be used as PCR template directly without extraction from the mixed solution. By using our kit, you can substantially shorten your time for digestion. Furthermore, the 2PCR Master Mix plus (With Dye) will guarantee accurate amplification consequence. Other sizes are also available. Please inqury us for quote.
Enzo Life Sciences AMPINEXT™ DNA Size Selection Kit (48Reactions)
The AMPINEXT™ DNA Size Selection Kit is a complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequencing applications. The kit utilizes magnetic bead technology and is suitable for Illumina, Life Technologies (SOLiD), Ion Torrent, and Roche/454 platforms. It can also be used for removing undesired larger DNA fragments by optimizing the bead to DNA volume ratio accordingly. The indicated number of reactions can be performed for a standard 50 µl solution input DNA sample. Assay Time: 30 minutes.
Agilent Technologies PFU ULTRA 2-HS2X MASTER
PFU Ultra II Hotstart PCR Master Mix is a 2X formulation of PfuUltra II Fusion HS DNA polymerase, an optimized PCR reaction buffer, magnesium, and dNTPs.