Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Alkali Scientific Radiant™ Green Hi-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Green Hi-ROX qPCR Kits, a high-performance 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ Green Hi-ROX qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection in combination with fluorescein for optional use with certain qPCR instruments.
System Biosciences LLC GLOBAL ULTRARAPID LENTIVIRAL TITER KITUltraRapid Lentiviral Global Titering Kit (Human and Mouse compatible) = 42 Reactions
Global UltraRapid™ Lentiviral Titering Kit - Rapidly determine pseudovirus titers by measuring the copy number of integrated lentiviral constructs. With a fast and simple workflow, the Global UltraRapid™ Lentiviral Titering Kit, delivers critical lentiviral titer information so you can move more quickly to the next step, whether that’s transduction of target cells in your own lab or delivery of packaged virus particles to another lab.
LUNA NANOTECH INC 8-Position Mini Magnetic Rack For 1.5 ML Tubes
- Convenient Mini format magnetic racks for fast separation of magnetic beads in 1.5 mL microcentrifuge tubes. These racks can be used with our PuroMAG Mini kits as well as various magnetic beads offered by Luna Nanotech and other manufactureres.
- Angled design brings the tube bottom closer to the magnet to reduce magnetic separation time.
- Removable magnet strip allows resuspension of magnetic beads without the need to take the tubes out of the rack.
LUNA NANOTECH INC Ngsprep Size Selection Magnetic Beads, 50 ML
PuroMAG™ NGSPrep Magnetic Beads, 50 mL
The PuroMAG™ NGSPrep Magnetic Beads are designed for fast and highly effective DNA clean-up after polymerase chain reaction (PCR, qPCR, ddPCR), microarrays, sequencing and other types of ezymatic reactions by removing unincorporated primers, dNTPs (deoxynucleotide triphosphates), salts, and other impurities. Combination of SPRI technology and paramagnetic feature of the beads allows for specific size selection of DNA fragments during the clean-up procedure with high recovery of the DNA product. This is crucial application in library preparation for various sequencing techniques, including next-generation sequencing (NGS).
Features
- Easy and fast clean-up of DNA PCR samples with efficient recovery of the product
- Highly efficient size selection of purified fragmented genome or PCR product for NGS library prep
- High throughput clean-up of 96/384 samples
- Can be used o
LUNA NANOTECH INC Dnase I, Rnase-Free, 5 X 550U
DNase I, RNase-Free, Lyophilized Powder, 5 x 550U
DNase I (Deoxyribonuclease I) is an endonuclease enzyme that can degrade DNA by cleaving phosphodiester linkages in the DNA backbone. DNase I is commonly used in RNA purification protocols to remove contaminating DNA.
This DNase I enzyme is RNase-free, supplied as lyophilized powder. Each tube contains DNAse I enzyme with a total activity of 550U (units).
LUNA NANOTECH INC Gel Extraction Kit, 50 Preps
PuroSPIN™ Gel Extraction Kit, 50 Preps
PuroSPIN™ Gel Extraction Kit is designed to provide simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose and PAGE gels. The purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acid binding to silica spin-columns, which allows efficient removal of gel residues and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as PCR, restriction digestion, ligation, labeling, or sequencing.
PuroSPIN™ columns can bind up to 200 μg of DNA product.
Features:
- Rapid purification of DNA fragments from standard agarose gels run in either T
Medchemexpress LLC 6-Carboxy-X-rhodamine | 194785-18-7 | 98.43% | 50 MG
6-ROX is a selective fluorescent probe and potential inhibitor of COX-2. It binds to the active site of COX-2 and inhibits its conversion of arachidonic acid into prostaglandins. This compound is frequently utilized in optical imaging applications related to tumors and inflammation, thereby assisting in the detection of diseased tissues that exhibit high expression levels of COX-2.
- Selective fluorescent probe and potential inhibitor of COX-2.
- Binds to the active site of COX-2.
- Inhibits conversion of arachidonic acid into prostaglandins.
- Used in optical imaging related to tumors and inflammation.
- Helps detect diseased tissues with high expression of COX-2.
LUNA NANOTECH INC Plasmid Miniprep Kit, 250 Preps
PuroSPIN™ Plasmid Miniprep Kit, 250 Preps
PuroSPIN™ Plasmid Miniprep Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.
Features:
- Easily purify plasmids from bacterial cultures using silica spin columns
- Simply extraction process with no need for phenol or chloroform
- Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
- Efficient removal