Cell Culture Reagents, Antibiotics and Supplements
Promocell GmbH PromoCell Macrophage Detachment Solution
Macrophages are strongly adherent and are not effectively dislodged from culture surfaces by standard enzymatic cell dissociation reagents. Indeed, these cells are highly sensitive to improper dissociation procedures. The PromoCell Macrophage Detachment Solution is chemically defined and non-enzymatic. It was especially designed for the gentle release of adherent macrophages and guarantees the best possible cell viability, even after prolonged exposure times. Unlike enzyme-based solutions, the PromoCell Macrophage Detachment Solution does not alter cell surface proteins and neutralization is not necessary.
Invivogen Plasmocure™ Antimycoplasma agent, 100 mg (1 ml)
Plasmocure: Mycoplasma Elimination Reagent. Product concentration: 100 mg/ml, Sterile ready-to-use solution, Elimination of mycoplasmas in two weeks (recommended concentration of 50 i1/4g/ml), Shipped at room temperature. Unit size 100 mg (1 ml)
Lonza Walkersville MycoAlert Mycoplasma Detection Kit (10 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. Benefits:Convenient and fastSimply add two reagents to the culture supernatantPerform two luminescence readingsResults in less than 20 minEasy interpretation of results Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma except Ureaplasma)Negligible interference with media componentsContent:2 x 600 µL MycoAlert Reagent (lyophilized)2 x 600 µL MycoAlert Substrate (lyophilized)1 x 10 mL MycoAlert Assay Buffer
InVitria ITSE Animal-Free - 10mL
InVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular signalingTransferrin: delivers intracellular iron via the transferrin receptor, drives cellular respiration, chelates free iron, and reduces generation of cytotoxic free radicals and peroxideSelenium: required for the activity of antioxidant enzymes such as glutathione peroxidase and thioredoxin reductaseEthanolamine: phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of certain cell typesAlbumin: binds, transports, and delivers ions, fatty acids, vitamins, hormones, small-molecule drugs, and toxins; regulates serum pH and osmotic pressure; and provides antioxidant activityMade in the USA.
Lifeline Cell Technology Gent/Ampho antibiotic solution
GA (Gentamicin Amphotericin B) 1000X, 0.5 mL. Antibiotic solution for cell culture.
Key Scientific Products CryoCare, Organism Preservative System, Yellow Beads, 50 Vials per Box
CryoCare organism preservative system lowers the cost of stock cultures by stretching each expensive culture into 15 or more which meet NCCLS standards. Vial contains ceramic beads in broth base. Includes 50 vials per box with yellow beads.
Lonza Walkersville MycoAlert Mycoplasma Detection Kit (50 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. Benefits:Convenient and fastSimply add two reagents to the culture supernatant Perform two luminescence readingsResults in less than 20 minutesDetects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma except Ureaplasma)Negligible interference with media componentsEasy interpretation of resultsContent:2 x 2.5 mL MycoAlert Reagent (lyophilized)2 x 2.5 mL MycoAlert Substrate (lyophilized)1 x 10 mL MycoAlert Assay Buffer
General Laboratory Products Saline (0.9%) - 100ml, 12 vials per case
Saline (0.9%) - 100ml. Saline is routinely used a diluent. This is an industrial use product, not for humans and not to be used as an injectable. 12 Vials per case. Used as a diluent.
Lonza Walkersville Trypsin/EDTA Solution, 100 mL
Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.Trypsin/EDTA is also a component of Lonza's ReagentPack Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.Content:1 x Bottle Trypsin/EDTA 100 mL
Invivogen Hygromycin B Gold, 1g
HygroGold, CAS No.: 31282-04-9, Molecular Formula: C20H37N3O13 HCl, Molecular Weight: 563.5, 1g liquid at 100mg/mL, Pack: 5 x 2mL/TB
HIMEDIA Acriflavin-Cefsulodin-Vancomycin
Supplement (ACV Supplement)
Acriflavin-Cefsulodin-Vancomycin Supplement (ACV Supplement)
STEMCELL Technologies StemSpan™ CD34+ Expansion Supplement (10X), Size: 10 mL
StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion of CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples.StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. This expansion may be further increased with the addition of small molecules such as UM729. See data tab for more details. StemSpan™ CD34+ Expansion Supplement (10X) is intended for use in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-AOF (Catalog #100-0130)