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Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. ACCUTASE™ does not contain mammalian or bacterial-derived products. Each lot of ACCUTASE™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
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NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) is recommended for the enzymatic digestion and dissociation of adult mouse and rat central nervous system (CNS) tissue. NeuroCult™ Enzymatic Dissociation Kit has been optimized so that the entire procedure is reproducible, fast, and yields high cell numbers and viabilities. The resulting single-cell suspension is ready for immediate use in downstream applications.
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Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 units per mg of protein.
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Trypsin Neutralizing Solution (TNS) is used to effectively neutralize Trypsin/EDTA. Lonza's TNS is availableindividually or as a component in our ReagentPack Subculture Reagents (CC-5034) which includes Trypsin and HEPES buffer.Content:1 x Bottle Trypsin Neutralizing Solution, 100 mL
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Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
ACCUTASE GMP Cell Detachment Solution is a non-mammalian, ready-to-use formulation designed to gently detach cells while maintaining their viability and surface marker integrity. Manufactured under GMP with rigorous testing for sterility and endotoxins, it eliminates the need for washing or neutralizing agents, thereby streamlining workflows in research and cell therapy applications. Its unique enzyme blend ensures high performance across various manufacturing setups.
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Trypsin is a serine protease produced in the pancreas. Ethylenediaminetetraacetic acid (EDTA) is a chelating agent which sequesters metal ions such as Ca+2 and Mg+2,enhancing the trypsinization reaction. 0.05% Trypsin/EDTA solution (T/E) is a sterile, phosphate and HEPES-buffered saline solution with a pH of 7.4 at room temperature. It contains 0.05% trypsin and 0.5 mM EDTA.
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