PAN-Biotech DMEM/F12 (1:1) Cell Culture Medium, w: L-Glutamine, w/o: Phenol red, w: 1.2 g/L NaHCO3. QUANTITY: 500 ml. MANUFACTURED BY: PAN-Biotech GmbH (Germany). BICARBONATE: With 1.2 g/L Sodium Bicarbonate. GLUCOSE: with glucose. GLUTAMINE: with L-Glutamine. HEPES: Without HEPES. PHENOL RED: Without Phenol Red. SODIUM PYRUVATE: with Sodium pyruvate. STERILE: Yes. STORAGE TEMPERATURE: +2-8°C. SUMMARY: DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cell cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components. Together, it combines DMEM's high concentration of amino acids and vitamins of DMEM and the higher concentration of zinc sulfate, putrescine and linoleic acid of F12.