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This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Basal salt mixture containing micro and macro elements as described by Murashige and Skoog (1962). MES buffer 500 mg/L added to prevent acidification during cultivation. Supplied as powder.
Concentration: 4802.1 mg/L
Hygroscopic
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TBS/TWEEN-20 (10X) 500 ML, IBB-580, 1% Tween-20 in 250 mM Tris-HCl, 27 mM KCl and 1.37 M NaCl, pH 7.4 +/- 0.2. Prepared in 18.2 megohm water.and autoclaved at 15 psi for 30 min on liquid cycle.
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