Additional Selective and Differential Cell Culture Media
Promocell GmbH PromoCell Mammary Epithelial Cell Growth Medium KIT 500 ml, Contains: C-21210, C-39110
The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for rat and murine mammary epithelial cells as well as mamma carcinoma cell lines.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml |
Promocell GmbH PromoCell Skeletal Muscle Cell Basal Medium 500 ml
The PromoCell Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Fetuin (bovine) | 50 μg / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 10 μg / ml || Dexamethasone | 0.4 μg / ml |
Promocell GmbH PromoCell Fibroblast Basal Medium 3 (prf) 500 ml, phenol red-free
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF).Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine cardiac fibroblasts.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Fibroblast Basal Medium 2 (prf) 500 ml, phenol red-free
PromoCell Fibroblast Growth Medium 2 is a low-serum (2% V/V) medium and has been developed for the in vitro cultivation of adult Human Fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.02 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Melanocyte Growth Medium KIT 500 ml, Contains: C-24210, C-39410
PromoCell Melanocyte Growth Medium is a PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M3.While the supplements of the Melanocyte Growth Medium are serum-free, the Basal Medium is manufactured with 0.2% serum.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Phorbol Myristate Acetate | 10 ng / ml |
Promocell GmbH PromoCell Small Airway Epithelial Cell Growth Medium 500 ml, ready-to-use, serum-free, with C-39175 attached to the flask
PromoCell Small Airway Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the distal respiratory system. The optimal adjustment of growth factors in this medium ensures excellent in vitro growth.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine small airway epithelial cells.| Media supplement | Final supplement concentration || Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml || Bovine Serum Albumin-Fatty Acid Free (BSA-FAF) | 2.5 mg/ml |
Promocell GmbH PromoCell Keratinocyte Basal Medium 2 (prf) 500 ml, calcium-free, phenol red-free
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |
Promocell GmbH PromoCell Keratinocyte Growth Medium 2 KIT 500 ml, Contains: C-20211, C-39011
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |
Promocell GmbH PromoCell Fibroblast Basal Medium (prf) 500 ml, phenol red-free
PromoCell Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vialsWhile the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.| Media supplement | Final supplement concentration|| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Renal Epithelial Cell Growth Medium 2 500 ml, ready-to-use, low-serum, with C-39606 attached to the flask
PromoCell Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine and mouse renal epithelial cells.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 36 ng / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 4 pg / ml || Transferrin (recombinant human) | 5 μg / ml |
General Laboratory Products Tryptic Soy Broth (TSB) w/4% Tween 20 & 0.5% Lecithin - 990ml | 6/cs
Tryptic Soy Broth (TSB) w/4% Tween 20 & 0.5% Lecithin - 990ml | 6/cs
ATHENA ES BEGM Medium
BEGM (Bronchial Epithelial Growth Medium) is supplied as a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors optimized for culturing initial harvest of human airway epithelial cells and for expansion of established cell lines. BEGM is derived from LHC Basal Medium which is supplemented with growth factors and supplements that eliminate the need for serum.
ATHENA ES VALI Medium
VALI (Vertex modified Air-Liquid Interface) medium is derived from ALI medium. The VALI version was developed to support drug-discovery using human bronchial epithelial cells in an air-liquid surface model
ATHENA ES DMEM Low Glucose
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Dulbecco's Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle's Minimal Essential Medium. Like the original DMEM formulation, this formulation contains a low concentration of glucose (1 g/L). DMEM also uses a sodium bicarbonate buffer system, allowing for maintenance of physiological pH in a 5–10% CO2 environment. Importantly, DMEM commonly requires fetal bovine serum (FBS) supplementation.
ATHENA ES DMEM/F12, basal media
Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM F12) is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. DMEM F12 is a 1:1 mixture of DMEM and Ham's F12 media. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with the wide variety of components found in Ham's F12 media.