General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay D1 Dopamine Receptor Frozen Cells, 2 Vials of Cells. (HTS102RTA)
The Ready-to-Assay D1 Dopamine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses DRD1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of DRD1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter CHO-K1 rCHRM4 β-Arrestin Cell Line, 2 vials. (93-0870C2)
The PathHunter rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen CCK1 Cholecystokinin Receptor Membrane Preparation, 1 vial. (HTS184M)
The ChemiScreen CCK1 Cholecystokinin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCKAR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay GPR103/Peptide P518 Receptor Frozen Cells, 2 Vials of Cells. (HTS189RTA)
The Ready-to-Assay GPR103/Peptide P518 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. QRFP neuropeptides) based activation of QRFPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses QRFPR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of QRFPR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Quantitative Genomic DNA from; Aspergillus flavus; Strain: SN 3
Quantitative Genomic DNA from Asperigillus flavus strain SN 3 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine fungal load.
Eurofins Discoverx PathHunter CHO-K1 MRGPRX1 β-Arrestin Cell Line, 2 vials. (93-0919C2)
The PathHunter MRGPRX1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. BAM) based activation of MRGPRX1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX1 and EA-tagged β-Arrestin-2. Activation of MRGPRX1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 VIPR2 β-Arrestin Cell Line, 2 vials. (93-0317C2)
The PathHunter VIPR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR149 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0420C2A)
The PathHunter GPR149 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR149 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR149 and EA-tagged β-Arrestin-2. Activation of GPR149 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Providencia rettgeri; Strain 1103204
Providencia rettgeri strain bMx# 1103204 is a whole-genome sequenced clinical isolate. The depositor states that this bacterium is a carbapenemase producer (OXA-48).
Biolegend PerCP/FireTM 780 anti-mouse
PerCP/Fire™ 780 anti-mouse CD223 (LAG-3) [C9B7W]; Isotype: Rat IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 ug
Innovative Research Inc Hmn PAI1 Rcmb WT Act 1 mg
Human PAI-1 Recombinant Wild Type Active 1 mg - Human PAI-1 Recombinant Wild Type Active from Innovative Research has been recombinantly produced in E. coli and purified by affinity chromatography. This is a Frozen liquid buffered in 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 and a purity of >95% as determined by SDS-PAGE. More Details Species Human Target PAI-1 Purification Method Affinity purified Purity >95% as determined by SDS-PAGE Analysis Source E. coli Storage Conditions -70C Additional Information Gentle purification conditions create an active fraction that is greater than 99% pure and 98% active. This product can be used as a control in applications like ELISA and Western Blot. It can be used in a wide range of in vitro applications, including blocking/inhibition, fibrinolysis, plasminogen activation, receptor binding, in hypertension studies, for protein-protein interactions, and more.
Eurofins Discoverx cAMP Hunter CHO-K1 RXFP3 (SALPR) Gi Cell Line, 2 vials. (95-0102C2)
The cAMP Hunter RXFP3 (SALPR) Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP3, a naturally Gi/Go coupled GPCR. Upon activation of RXFP3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ORGANABIO LLC Human Peripheral Blood Mononuclear Cells (PBMCs) / Cryopreserved / Single Donor / 15-23x vials of 1x10E8 cells/vial / OrganaBio LEUKOPAC - PBMC / Research Use Only
Human peripheral blood mononuclear cells (PBMCs) from recallable / HLA-typed healthy adult donors isolated by density gradient centrifugation. Comprehensive characterization including immunophenotyping (CD45 / CD14 / CD19 / CD56 / CD3 / CD3CD4 / CD3CD8) / infectious disease testing / and high-resolution HLA typing (Class I and II). Quarter leukopak processed into vials. Availble in 1X10E8 cells/vial. OrganaBio Human PBMCs are isolated from peripheral blood of normal / healthy adult donors by density gradient centrifugation. Each lot is cryopreserved and characterized by immunophenotyping. Comprehensive donor characterization includes infectious disease testing panel and high-resolution HLA typing with full donor demographics (age / sex / BMI / race / ethnicity / blood type).
Amsbio LLC A549 / Luciferase (Puromycin) stable cell line
A549 / Luciferase (Puromycin) stable cell line