General Cell Lines and Cell Cultures
ATCC Fusarium keratoplasticum; genomic DNA
Genomic DNA isolated from Fusarium keratoplasticum FIV/74. This fungal strain is also available as ATCC Catalog No.: 36031™.
ATCC Bacillus coagulans; Strain: IDCC 1201
Bacillus coagulans strain IDCC 1201 was isolated from green malt.
Eurofins Discoverx Ready-to-Assay α1B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS158RTA)
The Ready-to-Assay α1B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiBrite EP4 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS142L)
The ChemiBrite EP4 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER4, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen BB1 Bombesine/Neuromedin B Receptor Stable Cell Line, 2 vials. (HTS123C)
The ChemiScreen BB1 Bombesin/Neuromedin B Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay BB1 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS123RTA)
The Ready-to-Assay BB1 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS044M)
The ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen CCR9 Chemokine Receptor Membrane Preparation, 1 vial. (HTS036M)
The ChemiScreen CCR9 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR9 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Pfizer Pneumococcal polysaccharide powder Type 4 (d4) 10mg
Purified pneumococcal polysaccharide type 4 is manufactured by Pfizer, Inc. for small quantity distribution by ATCC. The pneumococcal powder type 4 is a component of the current 13-valent vaccine against Streptococcus pneumoniae. The pneumococcal polysaccharide type 4 can be used to assay for type-specific antibodies, research immune responses and study immunodeficiencies.
Eurofins Discoverx ChemiScreen CCR8 Chemokine Receptor Membrane Preparation, 1 vial. (HTS013M)
The ChemiScreen CCR8 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR8 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ACROBiosystems HEK293/Human ASGR1&ASGR2 Stable Cell Line
ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2).
ACROBiosystems HEK293/Human ErbB3 Stable Cell Line
ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.