DNA Reagents
Chemgenes Corporation Small Drying Traps, 10 pack, effective for 10-50 mL acetonitrile solutions, reduces moisture from 250 ppm to below 50 ppm, pre-activated, vacuum sealed
The Small Drying Traps are designed to maintain highly anhydrous environments essential for high-quality oligonucleotide synthesis. These traps effectively dry acetonitrile solutions ranging from 10-50 mL, significantly reducing moisture content from approximately 250 ppm to below 50 ppm. They come pre-activated and are packaged in vacuum-sealed foil packets for immediate usability. These traps are crucial for improving coupling efficiency during DNA/RNA synthesis, making them a vital part of laboratory workflows.
- Creates super anhydrous conditions critical for DNA/RNA synthesis
- Reduces moisture in acetonitrile from 250 ppm to below 50 ppm
- Supplied fully activated for immediate use
- Compatible with various acetonitrile-based solutions
- Enhances coupling yields during oligonucleotide synthesis
- Packaged in vacuum-sealed foil packets
- 10 traps per pack
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Bundle for Large Fragments – 20 reactions
NEB recommends NEB 10-beta Competent E. coli , High Efficiency for assemblies larger than 15 kb. NEBuilder HiFi DNA Assembly Bundle for Large Fragments offers a discount for purchasing these competent cells with the NEBuilder HiFi DNA Assembly Master Mix. NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-30 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.
New England Biolabs, Inc. NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) – 24 reactions
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext Small RNA Library Prep Set for Illumina (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.
NOVATIA LLC Oligo HTCS High Temp Trap Column, P/N HTCS-HTC1-4, 4 columns, max pressure 6000 PSI, elevated temperatures, compatible with Oligo HTCS LC/MS System, includes HTCS-HLDR-HT
The Novatia Oligo HTCS High Temp Trap Column is engineered for robust performance in HPLC applications, making it ideal for oligonucleotide high-throughput characterization systems. This column can withstand elevated temperatures and pressures up to 6,000 PSI, facilitating efficient online desalting and purification crucial for accurate oligonucleotide LC/HRMS analysis. Supplied as a convenient four-pack, it ensures continuity in high-demand scientific workflows.
- Designed for HPLC applications
- Maximum pressure rating of 6,000 PSI
- Suitable for elevated temperature conditions
- Pack includes 4 columns
- Compatible with Oligo HTCS LC/MS systems
- Requires Oligo HTCS High Temp Trap Holder
Takara Bio In-Fusion Snap Assembly Value Bundle | 50 rxns
In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes a NucleoSpin Gell and PCR Clean-Up Kit, Stellar Competent Cells, and PrimeSTAR Max DNA Polymerase.
Zymo Research Corporation EZ DNA Methylation-Lightning Kit (CE-IVD), 50 rxns
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
AA BLOCKS LLC Disodium carboxyethyl siliconate | 18191-40-7 | 196.14 g/mol | 5G
Disodium carboxyethyl siliconate | 18191-40-7 | 196.14 g/mol | 5G
LGC GENOMICS LLC (Lucigen) E. cloni EXPRESS BL21(DE3) Electrocompetent Cells, 12 RxnsGet better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
LucigensE. cloniEXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency ofE. cloniEXPRESS Electrocompetent Cells (>5 10^9cfu/g) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 10^7 cfu/g and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.