DNA Cloning and Mapping and Mutagenesis
Promega HaloTag™ Interchangeable Labeling Technology
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
LGC GENOMICS LLC (Lucigen) E. cloni EXPRESS BL21(DE3) Electrocompetent Cells, 12 RxnsGet better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
LucigensE. cloniEXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency ofE. cloniEXPRESS Electrocompetent Cells (>5 10^9cfu/g) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 10^7 cfu/g and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.
Promega Carboxy Flexi™ Cloning System
The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Promega HaloTag™ Mammalian Pull-Down and Labeling System
The HaloTag™ Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
Promega Single-Step (KRX) Competent Cells
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression with one strain.
LGC GENOMICS LLC (Lucigen) OverExpress ChemComboPack, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
Agilent Technologies BL21-GOLD(DE3)PLYSS C.CELLS
BL21-Gold (DE3)pLysS Competent Cells Provide high-level protein expression and Allow direct cloning of many expression constructs. Hte phenotype increases transformation efficiency to 1 x 108 of supercoiled DNA. Used for toxic proteins.
LGC GENOMICS LLC (Lucigen) CopyCutter EPI400 Chemically Competent E. coli, 10 Rxns
CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
Agilent Technologies BL21-CODONPLUS-RIL COMP CELLS
BL21-CodonPlus -RIL and -RP Competent Cells correct codon bias, dramatically improves expression when codon bias is a problem. Contains RIL strain for AT-rich genomes and proteins from all organisms.
ANTIBODY DESIGN LABS M13KO7 Helper Phage (concentrated), 1mL
M13KO7 is a helper phage designed for the production of single-stranded plasmid DNA (1). M13KO7 is an M13 phage that has the insertion of the origin of replication from p15A and the kanamycin-resistance gene from Tn 903 at the AvaI site at position 5825 of M13. M13KO7 does not contain the Met40Ile mutation in protein g2p as initially reported (1). Virions prepared with M13KO7 have a phagemid/helper ratio of around 9:1.
LGC GENOMICS LLC (Lucigen) Riboguard RNase Inhibitor, 10,000 units @ 40 U/LRNA is precious. Protect it with this high quality, effective RNase inhibitor. Manufactured under ISO-13485.
RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.RiboGuard RNase Inhibitor is the best defence against common RNases, including RNase A, RNase B, and RNase C. This recombinant RNase inhibitor protein provides reliable protection of valuable RNA samples by binding strongly to RNases in a 1:1 ratio. Advanced purification protocols and extensive quality control tests ensure RiboGuard RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.Applications: cDNA synthesis, RT-PCR, in vitro transcription and translationA potent affinity for RNases (Ki >10-14 M) ensures rapid inhibition even when trace amounts of RNase are present.Free of detectable RNase or DNase activity, and mammalian DNA.Does not interfere with enzymes commonly used to prepare or analyse RNA.Less sensitive to oxidation than traditional RNase inhibitors.
New England Biolabs, Inc. RecA – 200 µg
E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes. In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleoprotein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged.