Nucleic Acid Introduction Reagents
Cell Systems Corporation 5 Vials Primary Human Venous Endothelial Cells (HUVEC) - Same Lot (CSC2V0V5)
Boost your research program with 5 identical vials of passage 3 Primary Human Venous Endothelial Cells while saving ~ 20% on purchase. Isolated from the umbilical vein and, unlike cells from other providers, without positive control antibody labels. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact your Fisher Scientific Sales representative for tailored quotes. Tight Deadline? Our cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
Aat Bioquest Transfectamine 5000 Transfection Reagent (0.5 mL)
Transfectamine 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells or more specifically into animal cells. This catalog is for 0.5 mL.
Agilent Technologies TRANSPACK PACKAGING EXTRACT
Transpack Packaging Extract 100 vials Ideal for recovery of all lambda shuttle vectors Lack ofbeta-gal activity No artifacts affecting mutation data Highest recovery per extract reaction Deficient in McrA McrBC Mrr and McrF
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide | 28728-55-4 | MFCD00133397 | 100g
Hexadimethrine bromide | 95%MW: | 28728-55-4 | MFCD00133397 | 100g
Lonza Walkersville Human Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
DI TNC1 Rat Type 1 Astrocyte Cell Line (ATCC CRL-2005)
DI TNC1 (ATCC CRL-2005) is an immortalized rat type 1 astrocyte cell line established from primary cultures of diencephalon tissue of 1-day-old rats. Cells were transfected with an SV40 early region construct under the GFAP promoter and retain type 1 astrocyte characteristics such as GFAP immunoreactivity and high-affinity GABA uptake.
- Established from diencephalon tissue of 1-day-old rat neonates.
- Immortalized via SV40 early region under GFAP promoter.
- Retains GFAP immunoreactivity and type 1 astrocyte phenotype.
- High-affinity GABA uptake inhibitable by beta-alanine.
- Adherent growth suitable for monolayer culture.
- Culture in Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum at 37°C and 5% CO2.
- Cryopreserve in medium with 5% dimethyl sulfoxide; store in liquid nitrogen vapor.
- Mycoplasma not detected in tested stocks.