Transfection Reagents
Selleck Chemical LLC Ro-3306 10mg 872573-93-8
RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM siRNA Transf
Short interfering RNA (siRNA) can be directlyintroduced intocells by transfection. A lipid-based transfection reagent such as X-tremeGENE siRNA Transfection reagent can provide a convenient reliable and efficient vehicle for delivering siRNAs into animal cells enabling the study of cellular and functional consequences of gene knockdown.This innovative reagent forms a complex with siRNA as well as with mixtures of siRNA and plasmid DNA (cotransfection) and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps mix and incubate diluted transfection reagent with diluted siRNA then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity it can be used without media changes. The product is animal-component free.ContentsSOLUTIONon filtered through 0.2 mum pore size membrane supplied in polypropy
Agilent Technologies BL21CODONPLUS(DE3)RP C.CELLS
BL21-CodonPlus -RIL and -RP Competent Cells Correct codon bias, dramatically improves expression when codon bias is a problem, includes RIL strain for AT-rich genomes, RP strain for GC-rich genomes
Cell Systems Corporation Human Primary Keratinocytes (ACBRI 2K0)
Primary Human Primary Keratinocytes (ACBRI 2K0) were isolated from dispase dissociated dermal tissue. Unlike cells from other providers, Cell Systems primary cells are isolated without positive control antibody labels. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. These cells require specialized low-calcium Keratinocyte media. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact Fisher Scientific Sales rep for tailored quotes. Tight Deadline? Cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
OriGene Lentiviral Packaging Kits
Lenti-vpak packaging kit - packaging plasmids and transfection reagent, 5 packs.
Cell Biolabs Inc CytoSelect™ 96-Well Cell Transformation Assay Kit (Cell Recovery Compatible, Colorimetric), 96 assays
The traditional soft agar colony formation assay monitors anchorage-independent cell growth by measuring proliferation in a semisolid culture media in tissue culture dishes after 3-4 weeks, followed by manual counting of colonies. This method is cumbersome and not amenable to high throughput, and the manual counting of colonies is subjective because with varying colony sizes, it’s difficult to determine meaningful results. The CytoSelect™ 96-well Cell Transformation Assay (Cell Recovery Compatible) eliminates subjective manual counting of colonies with a much shorter assay time. Cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the provided colorimetric dye in a standard plate reader, making it a more quantitative and higher-throughput method. Additionally, the proprietary medium makes it possible to recover viable and intact cells for further downstream analysis. Each kit is sufficient for 96 tests in a microtiter plate.
Mayflower Bioscience LentiBOOST Research Grade Lentivirus Transduction Enhancer Solution, 200 Transductions
LentiBOOST is a chemical transduction enhancer simultaneously neutralizing electrostatic repulsion between cell membrane and virion and enhancing viral membrane fusion. In contrast to other chemical enhancers, LentiBOOST shows no toxic effects even in high concentrations. Therefore LentiBOOST is highly recommended for hard to transduce cells even in the presence of other transduction enhancers. LentiBOOST-R Research grade material is used for basic research only and is not transferable to the clinic.