Transfection Reagents
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM siRNA Transf
Short interfering RNA (siRNA) can be directlyintroduced intocells by transfection. A lipid-based transfection reagent such as X-tremeGENE siRNA Transfection reagent can provide a convenient reliable and efficient vehicle for delivering siRNAs into animal cells enabling the study of cellular and functional consequences of gene knockdown.This innovative reagent forms a complex with siRNA as well as with mixtures of siRNA and plasmid DNA (cotransfection) and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps mix and incubate diluted transfection reagent with diluted siRNA then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity it can be used without media changes. The product is animal-component free.ContentsSOLUTIONon filtered through 0.2 mum pore size membrane supplied in polypropy
Takara Bio XFECT TRANSFECTION REAGENT
Xfect Transfection reagent creates bio-degradable nanoparticles for superior transfection efficiency of plasmid DNA into mammalian cells.
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
New England Biolabs, Inc. pUC19 Vector – 50 µg
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases.