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Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.
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31 – 45 of 184 results
31

BD Human Pluripotent Stem Cell Transcription Factor Analysis Kit

Measuring expression of the stem cell master regulators'Nanog, Oct3/4, and Sox2'in heterogenous stem cell populations

32

BD Human and Mouse Pluripotent Stem Cell Analysis Kit

To analyze cells for expression of cell surface and intracellular pluripotency markers and differentiation markers

33

Promega RNasin™ Plus RNase Inhibitor

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Content And Storage -30°C to -10°C
Formulation Amount of RNasin Plus RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%
Concentration 40 U/μL
For Use With (Application) RT-PCR, quantitative RT-PCR, in-vitro transcription, in-vitro translation, coupled transcription/translation, useful in any applications where eukaryotic RNase contamination is a potential problem, protection of mRNA in cDNA synthesis reactions
34

Promega RiboMAX™ Large Scale RNA Production Systems

Designed for large-scale RNA production, the RiboMAX Systems consistently produce 2-5mg RNA from a 1ml reaction volume.

Includes Linear control DNA, RQ1 RNase-free DNase, Nuclease-free Water, Enzyme mix, rATP, rCTP, rGTP, rUTP, Sodium Acetate, 3m (pH 5.2), 5X Transcription Buffer
Content And Storage -30°C to -10°C
Quantity 1 System
For Use With (Application) Produce 2 to 5mg/mL of RNA in a 1mL reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe™ system transcription reaction
35

Promega Recombinant RNasin™ Ribonuclease Inhibitor

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Content And Storage -30°C to -10°C
Concentration 20 to 40 U/μL
For Use With (Application) RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems
36

Promega T7 RiboMAX™ Express Large Scale RNA Production System

The T7 RiboMAX Express Large Scale RNA Production System is an in vitro transcription system designed to produce milligram amounts of RNA in a short time.

37

Promega Transcend™ Nonradioactive Translation Detection Systems: IRNA

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

38

Promega TNT™ Quick Coupled Transcription/Translation System

The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.

Content And Storage <-65°C
For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
39

Promega Transcend™ Nonradioactive Translation Detection Systems

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

40

Promega T7 RiboMAX™ Express RNAi System

The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.

41

Promega Rabbit Reticulocyte Lysate, Untreated

Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

42

Promega RNasin™ Ribonuclease Inhibitor: Natural

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Formulation Amount of RNasin™ RNase inhibitor required to inhibit the activity of 5ng of RNase A by 50%, activity is measured by the inhibition of hydrolysis of Cytidine 2,3'-cyclic Monophosphate by RNase A
Concentration 20 to 40 U/μL
For Use With (Application) RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems, performing RNase-free Antibody staining, enhancement of RNA yields from Riboprobe™ system RNA synthesis reactions
43

Promega Riboprobe™ Systems

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

Content And Storage Store at -20°C.
Quantity 1 System
For Use With (Application) Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
44

Promega TNT™ T7 Quick Starter Bundle, Colorimetric

Purchase this bundle, and get the TNT T7 Quick Coupled Transcription/Translation System, your choice of Transcend Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTNT and pCMVTNT Vectors, at no extra cost.

45

Promega Rabbit Reticulocyte Lysate Systems, Nuclease Treated

Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their protein products and investigation of transcriptional and translational control.