Cell Based Assays
Invitrogen™ Total Reactive Oxygen Species (ROS) Assay Kit 520 nm
Contains the necessary regent and buffer for identifying ROS in cells by flow cytometry in the FITC channel. Reactive oxygen species are chemically reactive oxygen-containing molecules that are generated as a natural byproduct of the oxygen metabolism.
Invitrogen™ BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers
Dual-excitation ratiometric pH indicator
Invitrogen™ Mag-Fura-2, Tetrapotassium Salt, cell impermeant
Intracellular magnesium indicator that is ratiometric and UV light-excitable
Invitrogen™ Mag-Fluo-4, AM, cell permeant - Special Packaging
Useful as intracellular Mg2+ indicator as well as low-affinity Ca2+ indicator
Invitrogen™ Lipopolysaccharides From Escherichia coli Serotype 055:B5, Alexa Fluor™ 594 Conjugate
Bright, red-fluorescent conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes
Molecular Probes™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably, and quantitatively distinguish live and dead yeast in minutes by flow cytometry.
Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated (AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells
Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated, Alexa Fluor™ 594 Conjugate (Alexa Fluor™ 594 AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells
Invitrogen™ Dextran, Alexa Fluor™ 546; 10,000 MW, Anionic, Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Invitrogen™ FAST DiI™ oil; DiIΔ9,12-C18(3), ClO4 (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)
When applied to cells, dye diffuses laterally within the plasma membrane
Invitrogen™ Paclitaxel, Oregon Green™ 488 Conjugate (Oregon Green™ 488 Taxol, Flutax-2)
Probe for labeling tubulin filaments in live cells
Molecular Probes™ pHrodo™ AM Variety Pack
Quantify intracellular pH with the pHrodo AM Variety Pack, which contains pHrodo Red and Green AM intracellular pH indicator dyes that permeate cells and fluoresce in acidic cytosolic environments. These pHrodo AM dyes are compatible with HCS, HTS, fluorescence microplate readers, and flow cytometry.
Invitrogen™ Oregon Green™ 488 BAPTA-2, AM, cell permeant - Special Packaging
Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
Invitrogen™ Newport Green™ DCF Diacetate, cell permeant
Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+