Cell Based Assays
Promega ADP-Glo™ Kinase Assay + NEK3 Kinase Enzyme System
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a).
Promega ADP-Glo™ Kinase Assay + AMPK (A1/B1/G2) Kinase Enzyme System
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
Promega JNK1 Kinase Enzyme System, 1mg
Recombinant full-length human JNK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress.
Promega LRRK2 Kinase Enzyme System, 1mg
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
Promega Aurora A Kinase Enzyme System, 10microg
Recombinant full-length human Aurora A was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora A belongs to a multigenic family of mitotic serine/threonine kinases, which are involved in the control of chromosome segregation.
Promega PDGFR alpha Kinase Enzyme System, 1mg
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega CDK6/CyclinD3 Kinase Enzyme System, 10microg
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Molecular Probes™ SYTOX™ Green Dead Cell Stain, for flow cytometry
SYTOX™ Green Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays.
Thermo Scientific™ LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, rabbit
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement.
Thermo Scientific™ LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat
Contains Goat Tb-Anti-GST antibody
Promega NanoBiT PPI Control Pair (FKBP, FRB), 1 each
The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector. Starter systems also include the Nano-Glo Live Cell Assay System.
Promega NanoBRET TE Intracellular HDAC Complete Kit, 1,000 assays
NanoBRET Target Engagement Intracellular HDAC Assay measures compound binding at select HDAC target proteins within intact cells. The assay is based on NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.
Promega Corporation Lumit™ IL-2 (Human) Immunoassay
Perform a fast add-and-read protocol directly on cells with the No-Wash Immunoassay for IL-2 Detection. Luminescent detection with just a plate-reading luminometer, scalable to 96- or 384-well plates.
Alexa Fluor™ Kinase Tracer 314, 25 μM
Alexa Fluor™ Kinase Tracer 315 is detected by addition of a Eu-labeled anti-tag antibody.