Apoptosis Reagents and Kits
Enzo Life Sciences Caspase-2 fluorometric assay kit (25 tests)
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC. Applications: Fluorescent detection Activity assay.
Cytek Biosciences Inc FITC Annexin V
FITC Annexin V ..... Cat. No. 35-6409-T100 ..... Size 100 tests
Selleck Chemical LLC ML-7 HCl 10mg 110448-33-4
ML-7 is an inhibitor of smooth muscle myosin light chain kinase (MLCK) with a Ki value of 0.3 ¿M and displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. ML-7 also inhibits PKA and PKC with Ki of 21 ?M and 42 ?M, respectively. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Apexbio Technology LLC Annexin V-APC/DAPI Apoptosis Kit 200tests
Annexin V-APC/DAPI Apoptosis Kit allow discrimination between cells at various stages of apoptosis and cell death Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus
Apexbio Technology LLC Annexin V-PE/7-AAD Apoptosis Kit 200tests
Annexin V-PE/7-AAD Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes In addition this kit includes 7-AAD a nucleic acid dye that specifically binds double-stranded DNA and emits red fluorescence Normally 7-AAD cannot permeate intact cell membranes however it can penetrate compromised membranes of late apoptotic and dead cells staining their nuclei
ABclonal Technology Caspase-6 Rabbit mAb
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits large and small that dimerize to form the active enzyme This protein is processed by caspases 7 8 and 10 and is thought to function as a downstream enzyme in the caspase activation cascade Alternative splicing of this gene results in multiple transcript variants that encode different isoforms
ABclonal Technology Caspase-1 Rat mAb
Enables cysteine-type endopeptidase activity Involved in several processes including positive regulation of I-kappaB kinase NF-kappaB signaling positive regulation of interleukin-1 beta production and protein autoprocessing Acts upstream of or within several processes including membrane hyperpolarization mitochondrial depolarization and positive regulation of interleukin-1 alpha production Located in extracellular region Part of IPAF inflammasome complex Is expressed in knee joint and metanephros Orthologous to human CASP1 (caspase 1)
ABclonal Technology Annexin A6/Annexin VI
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways The annexin VI gene is approximately 60 kbp long and contains 26 exons It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths It is highly similar to human annexins I and II sequences each of which contain four such repeats Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis Alternatively spliced transcript variants have been described
Biolegend Ultra-LEAF™ Purified anti-human TNFSF18 (GITRL) [W21092F]; Isotype: Rat IgG2a, κ; Reactivity: Human; Apps: Block; Size: 100 μg
Ultra-LEAF™ Purified anti-human TNFSF18 (GITRL) [W21092F]; Isotype: Rat IgG2a, κ; Reactivity: Human; Apps: Block; Size: 100 μg
Cayman Chemical Z-YVAD-AFCtrIfluoroacetate 1mg
A fluorogenic substrate for caspase-1; AFC is released upon enzymatic cleavage by caspase-1 and its fluorescence can be used to quantify caspase-1 activity; ex/em = 400/505 nm, respectively