Signal Transduction Reagents and Kits
Promega CSK Kinase Enzyme System, 1mg
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
Promega PKC beta II Kinase Enzyme System, 1mg
Recombinant full-length human PKC beta II was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC beta II is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega PIP2:3PS Lipid Kinase Substrate, 0.25mg, 0.25ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.
Promega NUAK2 Kinase Enzyme System, 1mg
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
Promega ADP-Glo™ Kinase Assay + HIPK3 Kinase Enzyme System
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega MELK Kinase Enzyme System, 10microg
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega ASK1 Kinase Enzyme System, 1mg
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
Promega EGFR (L858R) Kinase Enzyme System, 1mg
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega TBK1 Kinase Enzyme System, 1mg
Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases.
Promega IGF1R Kinase Enzyme System, 1mg
Recombinant human IGF1R was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. IGF1R (Insulin-like Growth Factor 1) is a transmembrane tyrosine kinase receptor that is activated by IGF-1 and by the related growth factor IGF-2.
Promega ADP-Glo™ Kinase Assay + BRK Kinase Enzyme System
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega ADP-Glo™ Kinase Assay + EGFR (L861Q) Kinase Enzyme System
Recombinant human EGFR (L861Q) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay + MARK1 Kinase Enzyme System
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.
Promega FLT3 (D835Y) Kinase Enzyme System, 1mg
Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega IKK beta Kinase Enzyme System, 1mg
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.